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A DW echo planar imaging sequence with trace diffusion sensitization and b-values of 0 and 700 s/mm2, TR/TE = 7980 ms/84 ms, matrix size 128 × 128, FOV 237mm × 237mm, 64 slices and 1.86mm slice thickness, provided data for Apparent Diffusion Coefficient (ADC) maps. To estimate pre-contrast T1 relaxation time for DCE quantitation purposes, T1 mapping was performed by using a 3D FLASH sequence before the injection of contrast agent with TR/TE = 7.3 ms/4.41 ms, matrix size 128 × 128, FOV 230mm × 230mm, 20 slices, and 2.1mm slice thickness. This sequence was repeated five times at five different flip-angles of 2°, 5°,10°,15°, and 30°. DCE MRI utilized a 3D FLASH dual gradient-echo sequence with with TR/TE1/TE2/α = 6.8 ms/2.61 ms/3.89 ms/10°, matrix size 128 × 128, FOV 230mm × 230mm, 20 slices and 2.1mm slice thickness. The acquisition was repeated for 60 times (frames) for a total scan time of 6 min, corresponding to a temporal resolution of 6 s. A bolus of 0.1 mmol/kg of Gd-DTPA (gadopentetic acid) was injected 52 s after the scan started. Finally, a combined gradient-echo (ge) and spin-echo (se) 2D EPI sequence was performed to acquire DSC-MRI data with TR/TE(ge)/TE(se)/α = 1500 ms/31 ms/95 ms/80°, matrix size 160 × 160, FOV 192mm × 192mm, 12 slices and 5mm slice thickness. The acquisition was repeated 100 times for a total scan time of 2 min and 41 sec, corresponding to a temporal resolution of 1.61 s. 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They include two interactive databases linked together by de-identified tumor specimen numbers to facilitate comparisons across data modalities: • The open public image database on www.brain-map.org providing in situ hybridization (ISH) and RNA sequencing (RNA-Seq) data, which map gene expression across the anatomic structures and putative cancer stem cell clusters in glioblastoma. The associated histological dataset is annotated and is suitable for neuropathological examination. http://glioblastoma.alleninstitute.org/ • A companion database (Ivy GAP Clinical and Genomic Database) offering detailed clinical, genomic, and expression array data sets that are designed to elucidate the pathways involved in glioblastoma development and progression. This database requires registration for access. 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