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"@id": "https://doi.org/10.6084/m9.figshare.7981769.v1",
"url": "https://tandf.figshare.com/articles/Evaluation_of_an_i_in_vitro_i_coronary_stent_thrombosis_model_for_preclinical_assessment/7981769/1",
"additionalType": "Journal contribution",
"name": "Evaluation of an in vitro coronary stent thrombosis model for preclinical assessment",
"author": [
{
"name": "Dylan Perry-Nguyen",
"givenName": "Dylan",
"familyName": "Perry-Nguyen",
"@type": "Person"
},
{
"name": "Richard G. Jung",
"givenName": "Richard G.",
"familyName": "Jung",
"@type": "Person"
},
{
"name": "Alisha Labinaz",
"givenName": "Alisha",
"familyName": "Labinaz",
"@type": "Person"
},
{
"name": "Anne-Claire Duchez"
},
{
"name": "Omar Dewidar",
"givenName": "Omar",
"familyName": "Dewidar",
"@type": "Person"
},
{
"name": "Trevor Simard",
"givenName": "Trevor",
"familyName": "Simard",
"@type": "Person"
},
{
"name": "Denuja Karunakaran"
},
{
"name": "Kamran Majeed",
"givenName": "Kamran",
"familyName": "Majeed",
"@type": "Person"
},
{
"name": "Kiran Sarathy",
"givenName": "Kiran",
"familyName": "Sarathy",
"@type": "Person"
},
{
"name": "Ruonan Li"
},
{
"name": "F. Daniel Ramirez"
},
{
"name": "Pietro Di Santo",
"givenName": "Pietro Di",
"familyName": "Santo",
"@type": "Person"
},
{
"name": "Rebecca Rochman",
"givenName": "Rebecca",
"familyName": "Rochman",
"@type": "Person"
},
{
"name": "Derek So",
"givenName": "Derek",
"familyName": "So",
"@type": "Person"
},
{
"name": "Nicolas Foin",
"givenName": "Nicolas",
"familyName": "Foin",
"@type": "Person"
},
{
"name": "Benjamin Hibbert",
"givenName": "Benjamin",
"familyName": "Hibbert",
"@type": "Person"
}
],
"description": "Stent thrombosis remains an infrequent but significant complication following percutaneous coronary intervention. Preclinical models to rapidly screen and validate therapeutic compounds for efficacy are lacking. Herein, we describe a reproducible, high throughput and cost-effective method to evaluate candidate therapeutics and devices for either treatment or propensity to develop stent thrombosis in an in vitro bench-top model. Increasing degree of stent malapposition (0.00 mm, 0.10 mm, 0.25 mm and 0.50 mm) was associated with increasing thrombosis and luminal area occlusion (4.1 ± 0.5%, 6.3 ± 0.5%, 19.7 ± 4.5%, and 92.6 ± 7.4%, p < 0.0001, respectively). Differences in stent design in the form of bare-metal, drug-eluting, and bioresorbable vascular scaffolds demonstrated differences in stent thrombus burden (14.7 ± 3.8% vs. 20.5 ± 3.1% vs. 86.8 ± 5.3%, p < 0.01, respectively). Finally, thrombus burden was significantly reduced when healthy blood samples were incubated with Heparin, ASA/Ticagrelor (DAPT), and Heparin+DAPT compared to control (DMSO) at 4.1 ± 0.6%, 6.9 ± 1.7%, 4.5 ± 1.2%, and 12.1 ± 1.8%, respectively (p < 0.01). The reported model produces high throughput reproducible thrombosis results across a spectrum of antithrombotic agents, stent design, and degrees of apposition. Importantly, performance recapitulates clinical observations of antiplatelet/antithrombotic regimens as well as device and deployment characteristics. Accordingly, this model may serve as a screening tool for candidate therapies in preclinical evaluation.",
"license": "https://creativecommons.org/licenses/by/4.0/legalcode",
"keywords": "Biophysics, Biochemistry, Medicine, Physiology, FOS: Biological sciences, FOS: Biological sciences, Pharmacology, Biotechnology, 69999 Biological Sciences not elsewhere classified, 80699 Information Systems not elsewhere classified, FOS: Computer and information sciences, FOS: Computer and information sciences, Hematology",
"contentSize": "649794 Bytes",
"dateCreated": "2019-04-11",
"datePublished": "2019",
"dateModified": "2020-02-09",
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"@id": "https://doi.org/10.6084/m9.figshare.7981769",
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"isBasedOn": {
"@id": "https://doi.org/10.1080/09537104.2019.1595564",
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"schemaVersion": "http://datacite.org/schema/kernel-4",
"publisher": {
"@type": "Organization",
"name": "Taylor & Francis"
},
"provider": {
"@type": "Organization",
"name": "datacite"
}
}