10.6086/D1JQ21
Mauck, Kerry
0000-0001-9678-0619
University of California, Riverside
Chesnais, Quentin
0000-0002-0481-2335
National Research Institute for Agriculture, Food and Environment
Sun, Penglin
University of California, Riverside
Data for: Advanced infections by cucurbit yellow stunting disorder virus
encourage whitefly vector colonization while discouraging non-vector aphid
competitors
Dryad
dataset
2021
FOS: Agricultural sciences
United States Department of Agriculture
https://ror.org/01na82s61
CA-R-ENT-5144-H
California Department of Food and Agriculture
https://ror.org/04ma4gj04
18-0001-065-sc
California Melon Research Board*
2018-2020
2021-06-16T00:00:00Z
2021-06-16T00:00:00Z
en
162935 bytes
3
CC0 1.0 Universal (CC0 1.0) Public Domain Dedication
Plant viruses can change hosts in ways that increase vector contacts,
virion acquisition, and subsequent vector dispersal to susceptible hosts.
Based on this, researchers have proposed that virus-induced phenotypes are
the product of adaptations to “manipulate” hosts in ways that increase
transmission. Theoretical models of virus spread in crops support this
proposition; “manipulative” viruses spread faster and to a greater extent.
However, both empirical and theoretical studies on manipulation are
disproportionately focused on a few persistently transmitted pathogens,
and rarely consider the broader ecological implications of virus
infections . To address these knowledge gaps, we documented the effects of
different stages of infection by an economically devastating,
semi-persistently transmitted crinivirus, Cucurbit yellow stunting
disorder virus [CYSDV] on Cucumis melo (muskmelon) phenotypes, behavior
and performance of whitefly vectors (Bemisia tabaci) and non-vector aphid
competitors (Aphis gossypii). Whiteflies were strongly attracted to
CYSDV-infected hosts in a symptomatic stage of disease , but not in an
asymptomatic stage, and fed more easily on infected plants regardless of
symptom s . In contrast, aphids tended to avoid infected hosts, fed for
shorter periods of time, and produced fewer offspring on infected hosts .
Metabolomics revealed that host manipulations by CYSDV do not rely on
virus-induced shifts in leaf primary metabolites or volatiles but may
involve changes to phloem architecture and other compounds not measured
here . Our study demonstrates a sophisticated host manipulation by CYSDV,
whereby infection discourages colonization by a non-vector competitor
while inducing a suite of progressively more transmission-conducive
changes that encourage virion acquisition by the vector.
METHODOLOGICAL INFORMATION 1. Description of methods used for
collection/generation of data: This dataset was collected through
greenhouse and laboratory behavioral assays and laboratory chemical
analytical assays at the University of California, Riverside Department of
Entomology. 2. Methods for processing the data: Data were processed using
a series of generalized linear mixed models, non-parametric T-tests, and
multivariate analyses to produce a manuscript accepted for publication in
Journal of Pest Science. 3. Instrument- or software-specific information
needed to interpret the data: None. 4. Standards and calibration
information, if appropriate: None. 5. Environmental/experimental
conditions: Experiments were carried out at ambient temperatures that
ranged between 25 degrees Celsius and 29 degrees Celsius. 6. Describe any
quality-assurance procedures performed on the data: Data were hand-checked
by multiple researchers after entry. 7. People involved with sample
collection, processing, analysis and/or submission: Quentin Chesnais,
Penglin Sun, Kerry Mauck, Kristal Watrous.
Date of data collection: Approximately 2018-02-01 to 2020-02-01 Geographic
location of data collection: Riverside, CA 92521 SHARING/ACCESS
INFORMATION 1. Licenses/restrictions placed on the data: None 2. Links to
publications that cite or use the data:
https://doi.org/10.1007/s10340-021-01394-z 3. Links to other publicly
accessible locations of the data: None 4. Links/relationships to ancillary
data sets: None 5. Was data derived from another source? No 6. Recommended
citation for this dataset: Mauck, Kerry; Chesnais, Quentin; Sun, Penglin
(2021), Data for: Advanced infections by cucurbit yellow stunting disorder
virus encourage whitefly vector colonization while discouraging non-vector
aphid competitors, Dryad, Dataset, https://doi.org/10.6086/D1JQ21 DATA
& FILE OVERVIEW File List: The data are contained in a single
.xlsx file named “ALL_DATA.xlsx” with different datasets delineated by
tabs. Tabs contain a brief title description of the contents. In all tabs,
we use the following treatment codes: CYSDV 4wpi = Infected with cucurbit
yellow stunting disorder virus (CYSDV) and assayed at four weeks post
inoculation. CYSDV 2wpi = Infected with cucurbit yellow stunting disorder
virus (CYSDV) and assayed at two weeks post inoculation. Sham 4wpi =
Non-infected but exposed to whiteflies for a sham inoculation treatment
four weeks prior to assay time. Sham 2wpi = Non-infected but exposed to
whiteflies for a sham inoculation treatment two weeks prior to assay time.
Dataset descriptions by tab title: A. Btabaci 4way choice contact: Raw
counts and percentage responding to each treatment in four-way whitefly
(Bemisia tabaci MEAM1) choice tests where contact with plants was
permitted. B. Agossypii 2way choice contact: Raw counts and percentage
responding to each treatment in two-way aphid (Aphis gossypii) choice
tests where contact with plants was permitted. C. Btabaci 2way choice
volatiles: Raw counts and percentage responding to each treatment in
two-way whitefly choice tests where only volatile cues from plants were
permitted. S#-# and CY#-# designations indicate individual plants used as
the choices in each replicate. D. Btabaci EPG: Raw EPG data for
whiteflies. Treatment factors are infection status (virus) and time point
post inoculation (week). Response variables are total probing time (s Pr),
total time in pathway phase (s C), total time in the phloem salivation
phase (s E1), total time in phloem sap ingestion phase (s E2), time to
first phloem sap ingestion phase (t1E2), number of intracellular punctures
performed during pathway phase (pd). E. Agossypii EPG: Raw EPG data for
aphids. Treatment factors are infection status (virus) and time point post
inoculation (week). Response variables are total probing time (s Pr),
total time in pathway phase (s C), total time in the phloem salivation
phase (s E1), number of phloem sap ingestion phases (s E2), total time in
phloem sap ingestion phase (s E2), time to first phloem sap ingestion
phase (t1E2), number of intracellular punctures performed during pathway
phase (pd). F. Agossypii population growth: Aphid population counts after
11 days on infected and sham-inoculated plants. Treatment code refers to
the individual plant, Treatment refers to the infection status (infected
plants were between two and four weeks post-inoculation), Block refers to
the experiment replication, and Aphid Number is the number of individual
aphids on the plant after 11 days of population growth. G. Btabaci
oviposition: Whitefly egg deposition over a three day period of caging on
plants. At left, data for 4wpi, at right data for 2wpi. Plant refers to
the infection treatment, Eggs refers to the number of whitefly eggs
deposited over 3 days, Repetition refers to the experiment replicate (A or
B), and Leaf refers to the leaf used A=4th leaf from the crown, B = 5th
leaf from the crown. H. Amino Acids: Quantities of amino acids, in
micrograms per gram of leaf tissue. Sample name refers to the individual
plants, Virus Treatment refers to the infection status, Time Point refers
to the weeks post CYSDV inoculation or sham inoculation (two or four), and
Leaf Position refers to the sampling location (upper leaf #7 or 8, or
lower leaf near the crown, usually leaf #4). Amino acid column headers are
the response variables. I. Sugars: Data as for “H” above, but with sugars
as response variables. Amounts are corrected for plant weight. J.
Volatiles: Odor emissions from 4wpi infected and sham-inoculated plants.
Sample Name refers to the individual plants used, Treatment refers to the
infection status, Session refers to the replication of the experiment.
Response variables are individual volatile compounds. K. Leaf color: Data
on whitefly settling on individual plant leaves from four-way choice
tests. Box refers to the test arena used, Settling at 24h refers to the
number of whiteflies arrested on the plant assayed for color, and % yellow
is the reflectance of yellow wavelengths of light from the leaf surface.