10.6084/M9.FIGSHARE.C.6252791.V1
Xingyu Rao
Xingyu
Rao
Children's Hospital of Suzhou University
First Affiliated Hospital of Gannan Medical University
Heting Dong
Heting
Dong
Children's Hospital of Suzhou University
Weili Zhang
Weili
Zhang
Children's Hospital of Suzhou University
Huiming Sun
Huiming
Sun
Children's Hospital of Suzhou University
Wenjing Gu
Wenjing
Gu
Children's Hospital of Suzhou University
Xinxing Zhang
Xinxing
Zhang
Children's Hospital of Suzhou University
Li Huang
Li
Huang
Children's Hospital of Suzhou University
Yongdong Yan
Yongdong
Yan
Children's Hospital of Suzhou University
Chuangli Hao
Chuangli
Hao
Children's Hospital of Suzhou University
Wei Ji
Wei
Ji
Children's Hospital of Suzhou University
Canhong Zhu
Canhong
Zhu
Children's Hospital of Suzhou University
Zhengrong Chen
Zhengrong
Chen
Children's Hospital of Suzhou University
MiR-493-5p inhibits Th9 cell differentiation in allergic asthma by targeting FOXO1
Abstract The role of micro RNAs (miRNAs) in asthma remains unclear. In this study, we examined the role of miRNA in targeting FOXO1 in asthma. Results showed that miR-493-5p was one of the differentially expressed miRNAs in the PBMCs of asthmatic children, and was also associated with Th cell differentiation. The miR-493-5p expression decreased significantly in the OVA-induced asthma mice than the control groups. The miR-493-5p mimic inhibited the expression of the IL-9, IRF4 and FOXO1, while the inhibitor restored these effects. Moreover, the Dual-Luciferase analysis results showed FOXO1 as a novel valid target of miR-493-5p. According to the rescue experiment, miR-493-5p inhibited Th9 cell differentiation by targeting FOXO1. Then the exosomes in association with the pathogenesis of asthma was identified. Various inflammatory cells implicated in asthmatic processes including B and T lymphocytes, DCs, mast cells, and epithelial cells can release exosomes. Our results demonstrated that the DC-derived exosomes can inhibit Th9 cell differentiation through miR-493-5p, thus DC-derived exosomal miR-493-5p/FOXO1/Th9 may serve as a potential therapeutic target in the development of asthma.
Biophysics
Biochemistry
Cell Biology
Genetics
Molecular Biology
Pharmacology
Chemical Sciences not elsewhere classified
Immunology
Biological Sciences not elsewhere classified
Developmental Biology
Cancer
Science Policy
Hematology
Virology
figshare
2022
2022-10-18
2022-10-18
Collection
10.6084/m9.figshare.c.6252791
CC BY 4.0