10.6084/M9.FIGSHARE.C.6250555
Jia Li
Jia
Li
Ministry of Education of the People's Republic of China
Wei Xin
Wei
Xin
Ministry of Education of the People's Republic of China
Weiping Wang
Weiping
Wang
Ministry of Education of the People's Republic of China
Shijiao Zhao
Shijiao
Zhao
Ministry of Education of the People's Republic of China
Lu Xu
Lu
Xu
Ministry of Education of the People's Republic of China
Xingdong Jiang
Xingdong
Jiang
Ministry of Education of the People's Republic of China
Yuxuan Duan
Yuxuan
Duan
Ministry of Education of the People's Republic of China
Hongliang Zheng
Hongliang
Zheng
Ministry of Education of the People's Republic of China
Luomiao Yang
Luomiao
Yang
Ministry of Education of the People's Republic of China
Hualong Liu
Hualong
Liu
Ministry of Education of the People's Republic of China
Yan Jia
Yan
Jia
Ministry of Education of the People's Republic of China
Detang Zou
Detang
Zou
Ministry of Education of the People's Republic of China
Jingguo Wang
Jingguo
Wang
Ministry of Education of the People's Republic of China
Mapping of Candidate Genes in Response to Low Nitrogen in Rice Seedlings
Abstract Nitrogen is not only a macronutrient essential for crop growth and development, but also one of the most critical nutrients in farmland ecosystem. Insufficient nitrogen supply will lead to crop yield reduction, while excessive application of nitrogen fertilizer will cause agricultural and eco-environment damage. Therefore, mining low-nitrogen tolerant rice genes and improving nitrogen use efficiency are of great significance to the sustainable development of agriculture. This study was conducted by Genome-wide association study on a basis of two root morphological traits (root length and root diameter) and 788,396 SNPs of a natural population of 295 rice varieties. The transcriptome of low-nitrogen tolerant variety (Longjing 31) and low-nitrogen sensitive variety (Songjing 10) were sequenced between low and high nitrogen treatments. A total of 35 QTLs containing 493 genes were mapped. 3085 differential expressed genes were identified. Among these 493 genes, 174 genes showed different haplotype patterns. There were significant phenotype differences among different haplotypes of 58 genes with haplotype differences. These 58 genes were hypothesized as candidate genes for low nitrogen tolerance related to root morphology. Finally, six genes (Os07g0471300, Os11g0230400, Os11g0229300, Os11g0229400, Os11g0618300 and Os11g0229333) which expressed differentially in Longjing 31 were defined as more valuable candidate genes for low-nitrogen tolerance. The results revealed the response characteristics of rice to low-nitrogen, and provided insights into regulatory mechanisms of rice to nitrogen deficiency.
Genetics
Plant Biology
figshare
2022
2022-10-17
2022-10-17
Collection
CC BY 4.0