10.6084/M9.FIGSHARE.C.6249637.V1
Hongrui Jiang
Hongrui
Jiang
Chinese Academy of Sciences
Yan Ren
Yan
Ren
Chinese Academy of Sciences
Junyao Guo
Junyao
Guo
Chinese Academy of Sciences
Huijie Yang
Huijie
Yang
Chinese Academy of Sciences
Xiaotong Zhu
Xiaotong
Zhu
Chinese Academy of Sciences
Wenhao Li
Wenhao
Li
Chinese Academy of Sciences
Liangzhi Tao
Liangzhi
Tao
Chinese Academy of Sciences
Yue Zhan
Yue
Zhan
Chinese Academy of Sciences
Qi Wang
Qi
Wang
Chinese Academy of Sciences
Yuejin Wu
Yuejin
Wu
Chinese Academy of Sciences
Binmei Liu
Binmei
Liu
Chinese Academy of Sciences
Yafeng Ye
Yafeng
Ye
Chinese Academy of Sciences
CEF3 is involved in membrane trafficking and essential for secondary cell wall biosynthesis and its mutation enhanced biomass enzymatic saccharification in rice
Abstract Background As one of the most important staple food crops, rice produces large of agronomic biomass residues that contain lots of secondary cell walls (SCWs). Membrane trafficking plays key roles in SCWs biosynthesis, but information association membrane trafficking and SCWs formation in plants is limited. Results In this study, we report the function characterization of a rice mutant, culm easily fragile 3 (cef3), that exhibits growth retardation and fragile culm phenotype with significantly altered cell wall composition and reduced secondary wall thickness. Map-based cloning revealed that CEF3 encodes a homologous protein of Arabidopsis STOMATAL CYTOKINESIS DEFECTIVE2 (SCD2). The saccharification assays revealed that CEF3 mutation can improve biomass enzymatic saccharification. Expression pattern analysis indicated that CEF3 is ubiquitously expressed in many organs at different developmental stages. Subcellular localization revealed that CEF3 is a Golgi-localized protein. The FM4-64 uptake assay revealed CEF3 is involved in endocytosis. Furthermore, mutation of CEF3 not only affected cellulose synthesis-related genes expression, but also altered the abundance of cellulose synthase catalytic subunit 9 (OsCESA9) in the PM and in the endomembrane systems. Conclusions This study has demonstrated that CEF3 participates in the membrane trafficking that is essential for normal cellulose and other polysaccharides biosynthesis of the secondary cell wall, thereby manipulation of CEF3 could alter cellulose content and enhance biomass enzymatic saccharification in rice plants. Therefore, the study of the function of CEF3 can provide a strategy for genetic modification of SCWs in bioenergy crops.
Genetics
Plant Biology
figshare
2022
2022-10-15
2022-10-15
Collection
10.6084/m9.figshare.c.6249637
CC BY 4.0