10.6084/M9.FIGSHARE.C.6169263.V2
Charles O. Nwonuma
Charles O.
Nwonuma
0000-0001-8930-055X
Landmark University
Victoria C. Nwatu
Victoria C.
Nwatu
Landmark University
Gomaa Mostafa-Hedeab
Gomaa
Mostafa-Hedeab
Beni-Suef University
Al Jouf University
Oluyomi S. Adeyemi
Oluyomi S.
Adeyemi
Landmark University
Omokolade O. Alejolowo
Omokolade O.
Alejolowo
Landmark University
Oluwafemi Adeleke Ojo
Oluwafemi Adeleke
Ojo
Bowen University
Landmark University
Sylvanus A. Adah
Sylvanus A.
Adah
University of Ilorin
Oluwakemi J. Awakan
Oluwakemi J.
Awakan
Landmark University
Charles E. Okolie
Charles E.
Okolie
Elizade University
Landmark University
Nnaemeka Tobechukwu Asogwa
Nnaemeka Tobechukwu
Asogwa
Inemesit A. Udofia
Inemesit A.
Udofia
University of Lagos
Godshelp O. Egharevba
Godshelp O.
Egharevba
Landmark University
Nada H. Aljarba
Nada H.
Aljarba
Princess Nourah bint Abdulrahman University
King Saud University
Saad Alkahtani
Saad
Alkahtani
King Saud University
Gaber El-Saber Batiha
Gaber El-Saber
Batiha
Damanhour University
Experimental validation and molecular docking to explore the active components of cannabis in testicular function and sperm quality modulations in rats
Abstract Background Data available support that ninety percent of male infertility cases are due to low sperm counts. There is a scarcity of data on the medicinal effects of cannabis on fertility. This study evaluated testicular function and sperm quality modulation with cannabis in rats. Methodology Twenty-five male Wistar rats were randomly grouped into five: A, B, C, and D, each group have 5 rats. A (control): 0.2 ml 2% DMSO, B (vitamin C): 90 mg/kg body weight, C, D, and E were administered: 5 mg/kg, 10 mg/kg and 20 mg/kg body weight of ethanolic leaf extract of cannabis (ELEC) respectively. The rats were sacrificed 24 h after the last day of the 60 day oral administrations. Flavonoids were the predominant phytochemical present in the extract while quercetin, kemferol, silyman and gallic acid were identified. Results The results showed a significant improvement (p < 0.05) in sperm quality and a significant increase in the concentrations of follicle-stimulating hormone, luteinizing hormone, triglycerides, cholesterol, and total protein determination compared to the normal control. Similarly, there was a significant increase (p < 0.05) in the activities of acid phosphatase, alkaline phosphatase, and superoxide dismutase compared to the normal control. RAC-alpha serine/threonine-protein kinase (AKT1)-silymarin complexes (-8.30 kcal/mol) and androgen receptor (AR)-quercetin complexes (9.20 kcal/mol) had the highest affinity. Conclusion The antioxidant effects of the flavonoids in the ethanolic extract of cannabis may have protected testicular and sperm cells from oxidative damage. Biochemical processes and histopathological morphology were preserved by cannabis. The docking prediction suggests that the bioactive principle of cannabis may activate the androgenic receptors. The androgenic receptor modulation may be attributed to silymarin and quercetin.
Biochemistry
Medicine
Cell Biology
Genetics
Neuroscience
Physiology
Pharmacology
Biotechnology
39999 Chemical Sciences not elsewhere classified
Developmental Biology
111714 Mental Health
figshare
2022
2022-10-15
2022-10-15
Collection
10.1186/s12906-022-03704-z
10.6084/m9.figshare.c.6169263
CC BY 4.0