10.6084/M9.FIGSHARE.7855583
Nicholas R. Anderson
Alexander Buffone Jr.
Daniel A. Hammer
T lymphocytes migrate upstream after completing the leukocyte adhesion cascade
<p>The leukocyte adhesion cascade is of critical importance for both the maintenance of immune homeostasis and the ability of immune cells to perform effector functions. Here, we present data showing CD4<sup>+</sup> T cells migrate upstream (against the direction of flow) after completing the leukocyte adhesion cascade on surfaces displaying either ICAM-1 or ICAM-1 and VCAM-1, but migrate downstream on surfaces displaying only VCAM-1. Cells completing the cascade on HUVECs initially migrate upstream before reverting to more random migration, partly caused by transmigration of cells migrating against the flow. Furthermore, cells migrating upstream transmigrate faster than cells migrating downstream. On HUVECs, blocking interactions between LFA-1 and ICAM-1 resulted in downstream migration and slower transmigration. These results further suggest a possible physiological role for upstream migration <i>in vivo</i>.</p>
Biophysics
Microbiology
Cell Biology
Molecular Biology
Sociology
39999 Chemical Sciences not elsewhere classified
Ecology
Immunology
Developmental Biology
Cancer
Hematology
Computational Biology
Taylor & Francis
2019
2019-03-17
2019-12-18
Dataset
12999198 Bytes
10.1080/19336918.2019.1587269
CC BY 4.0