10.6084/M9.FIGSHARE.6109226
Cuicui Lyu
Cuicui
Lyu
Jun Shen
Jun
Shen
Rui Wang
Rui
Wang
Haihui Gu
Haihui
Gu
Jianping Zhang
Jianping
Zhang
Feng Xue
Feng
Xue
Xiaofan Liu
Xiaofan
Liu
Wei Liu
Wei
Liu
Rongfeng Fu
Rongfeng
Fu
Liyan Zhang
Liyan
Zhang
Huiyuan Li
Huiyuan
Li
Xiaobing Zhang
Xiaobing
Zhang
Tao Cheng
Tao
Cheng
Renchi Yang
Renchi
Yang
Lei Zhang
Lei
Zhang
Additional file 6: of Targeted genome engineering in human induced pluripotent stem cells from patients with hemophilia B using the CRISPR-Cas9 system
Figure S3. showing off-target effects detection in successful inserted iPSCs. Using Cas-OFFinder, 1799 potential off-target sites that differed from the sgRNA sequence by up to five nucleotides in the genome were found. We found 97,968 indels, 3084 SVs, 51,628 SNPs, and 2225 CNVs unique to the inserted iPSCs compared to that in the parental iPSCs. Since indels and SVs comprise virtually all of the mutations introduced by CRISPR-Cas9, we focused solely on indels and SVs. Through comparison of potential off-target sites, and indels and SVs unique to the inserted iPSCs, we found no overlapping mutation between them. (DOCX 302 kb)
Space Science
Microbiology
Genetics
Molecular Biology
Evolutionary Biology
Ecology
Biological Sciences not elsewhere classified
Information Systems not elsewhere classified
figshare
2018
2018-04-07
2023-06-13
Journal contribution
310267 Bytes
10.1186/s13287-018-0839-8
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