10.6084/M9.FIGSHARE.6073331.V1
Jun Chen
Jun
Chen
Jianwei Chen
Jianwei
Chen
Sijia Wang
Sijia
Wang
Guangmin Zhou
Guangmin
Zhou
Danqing Chen
Danqing
Chen
Huawei Zhang
Huawei
Zhang
Hong Wang
Hong
Wang
Development and validation of polar RP-HPLC method for screening for ectoine high-yield strains in marine bacteria with green chemistry
<p>A novel, green, rapid, and precise polar RP-HPLC method has been successfully developed and screened for ectoine high-yield strain in marine bacteria. Ectoine is a polar and extremely useful solute which allows microorganisms to survive in extreme environmental salinity. This paper describes a polar-HPLC method employed polar RP-C18 (5 μm, 250 × 4.6 mm) using pure water as the mobile phase and a column temperature of 30 °C, coupled with a flow rate at 1.0 mL/min and detected under a UV detector at wavelength of 210 nm. Our method validation demonstrates excellent linearity (<i>R</i>
<sup>2</sup> = 0.9993), accuracy (100.55%), and a limit of detection LOQ and LOD of 0.372 and 0.123 μgmL<sup>−1</sup>, respectively. These results clearly indicate that the developed polar RP-HPLC method for the separation and determination of ectoine is superior to earlier protocols.</p>
Biochemistry
Space Science
Environmental Sciences not elsewhere classified
Immunology
Biological Sciences not elsewhere classified
Marine Biology
Inorganic Chemistry
Taylor & Francis
2018
2018-04-02
2024-02-16
Journal contribution
161710 Bytes
10.6084/m9.figshare.6073331
10.1080/14786419.2018.1457668
CC BY 4.0