10.6084/M9.FIGSHARE.5969338.V1
Gaia Favero
Anna Paini
Carolina De Ciuceis
Luigi F. Rodella
Enrico Moretti
Enzo Porteri
Claudia Rossini
Silvia Ministrini
Leonardo Solaini
Caletti Stefano
Maria Antonietta Coschignano
Valeria Brami
Alina Petelca
Matteo Nardin
Ilenia Valli
Guido A. M. Tiberio
Francesca Bonomini
Claudia Agabiti Rosei
Nazario Portolani
Damiano Rizzoni
Rita Rezzani
Changes in extracellular matrix in subcutaneous small resistance arteries of patients with essential hypertension
<p><b>Background:</b> In the development of hypertensive microvascular remodeling, a relevant role may be played by changes in extracellular matrix proteins. Aim of this study was the to evaluate some extracellular matrix components within the tunica media of subcutaneous small arteries in 9 normotensive subjects and 12 essential hypertensive patients, submitted to a biopsy of subcutaneous fat from the gluteal or the anterior abdominal region.</p> <p><b>Patients and Methods:</b> Subcutaneous small resistance arteries were dissected and mounted on an isometric myograph, and the tunica media to internal lumen ratio was measured. In addition, fibronectin, laminin, transforming growth factor-beta-1 (TGF-β1) and emilin-1 contents within the tunica media were evaluated by immunofluorescence and relative immunomorphometrical analysis (immunopositivity % of area). The total collagen content and collagen subtypes within the tunica media were evaluated using both Sirius red staining (under polarized light) and immunofluorescence assay.</p> <p><b>Results:</b> Normotensive controls had less total and type III collagen in respect with hypertensive patients. Fibronectin and TGF-β1 tunica media content was significantly greater in essential hypertensive patients, compared with normotensive controls, while laminin and emilin-1 tunica media content was lesser in essential hypertensive patients, compared with normotensive controls. A significant correlation was observed between fibronectin tunica media content and media to lumen ratio.</p> <p><b>Conclusions:</b> Our results indicate that, in small resistance arteries of patients with essential hypertension, a relevant fibrosis may be detected; fibronectin and TGF-β1 tunica media content is increased, while laminin and emilin-1 content is decreased; these changes might be involved in the development of small resistance artery remodeling in humans.</p>
Biochemistry
Space Science
Medicine
Cell Biology
Genetics
Biotechnology
39999 Chemical Sciences not elsewhere classified
Immunology
69999 Biological Sciences not elsewhere classified
Developmental Biology
Cancer
Taylor & Francis
2018
2018-03-10
2018-07-03
Journal contribution
201728 Bytes
10.1080/08037051.2018.1448256
10.6084/m9.figshare.5969338
CC BY 4.0