10.6084/M9.FIGSHARE.21856602.V1
Yuxuan Jiang
Yuxuan
Jiang
Xiaoping Yin
Xiaoping
Yin
Qian Xu
Qian
Xu
Air Force Medical University
Southeast University
Shandong University
Qingdao University
Guangzhou University of Chinese Medicine
First Hospital of China Medical University
Xiangya Hospital Central South University
Zhongda Hospital Southeast University
Jilin University
Sichuan Agricultural University
Xiaoxiao Tang
Xiaoxiao
Tang
Huajie Zhang
Huajie
Zhang
Jing Lin
Jing
Lin
Sun Yat-sen University
Central South University
Fujian Agriculture and Forestry University
Southwest Medical University
Shantou University Medical College
First Affiliated Hospital of Shantou University Medical College
First Affiliated Hospital of Wenzhou Medical University
SingHealth
Jiangsu Academy of Agricultural Sciences
Shenzhen University Health Science Center
First Institute of Oceanography
Fudan University
Yi Wang
Yi
Wang
Zhejiang University
Chongqing University
Johns Hopkins University
China Agricultural University
Tongji University
Jiangnan University
Soochow University
Wenzhou Medical University
Shandong University
University of California, Davis
Nanjing Agricultural University
Huazhong University of Science and Technology
Tianjin University
Nanjing University of Science and Technology
Jishou University
Shanghai First People's Hospital
Shanghai Tenth People's Hospital
Shanghai University of Traditional Chinese Medicine
Zhongnan Hospital of Wuhan University
Beijing Proteome Research Center
National University of Singapore
Nanjing Drum Tower Hospital
Affiliated Hospital of Nantong University
Zhejiang A & F University
Second Hospital of Anhui Medical University
Second Xiangya Hospital of Central South University
Jilin Agricultural University
Shangrao Normal University
Zhejiang University of Technology
University of Electronic Science and Technology of China
Sichuan Agricultural University
Fudan University
Fei Yang
Fei
Yang
Zhejiang University
University of Arizona
Central South University
Yangtze University
Yunnan Agricultural University
Hebei North University
Third Affiliated Hospital of Sun Yat-sen University
NaseerUllah Khan
NaseerUllah
Khan
Liming Shen
Liming
Shen
Danqing Zhao
Danqing
Zhao
SWATH Proteomics analysis of placental tissue with intrahepatic cholestasis of pregnancy_part1
<p> </p>
<p><strong>Introduction:</strong> Intrahepatic cholestasis of pregnancy (ICP) usually occurs in the second and third trimesters. The disease's etiology and diagnostic criteria are currently unknown. Based on a sequence window to obtain all theoretical fragment ions (SWATH) proteomic approach, this study sought to identify potential proteins in placental tissue that may be involved in the pathogenesis of ICP and adverse fetal pregnancy outcomes.</p>
<p><strong>Methods:</strong> The postpartum placental tissue of pregnant women with ICP were chosen as the case group (ICP group) (subdivided into mild ICP group (MICP group) and severe ICP group (SICP group)), and healthy pregnant women were chosen as the control group (CTR). The SWATH analysis combined with liquid chromatography-tandem mass spectrometry (LC-MS) was used to screen the differentially expressed proteins (DEPs) in ICP and CTR groups, and bioinformatics analysis was used to find out the biological process of these differential proteins. </p>
<p><strong>Results:</strong> Proteomic studies showed there were 126 DEPs from pregnant women with ICP and healthy pregnant women. Most of the identified proteins were functionally related to humoral immune response, cell response to lipopolysaccharide, antioxidant activity and heme metabolism. A subsequent examination of placentas from patients with mild and severe ICP revealed 48 proteins that were differentially expressed. Through death domain receptors and fibrinogen complexes, these DEPs primarily regulate extrinsic apoptotic signaling pathways, blood coagulation, and fibrin clot formation. </p>
<p><strong>Discussion: </strong>This preliminary study helps us to understand the changes in the placental proteome of ICP patients, and provides new insights into the pathophysiology of ICP.</p>
Proteomics and intermolecular interactions (excl. medical proteomics)
figshare
2023
2023-01-12
2023-01-12
Online resource
15728640000 Bytes
10.6084/m9.figshare.21856602
CC BY 4.0