10.6084/M9.FIGSHARE.21601415.V1
Albrecht Fröhlich
Albrecht
Fröhlich
University of Ulm
Florian Olde Heuvel
Florian
Olde Heuvel
University of Ulm
Rida Rehman
Rida
Rehman
University of Ulm
Sruthi Sankari Krishnamurthy
Sruthi Sankari
Krishnamurthy
University of Ulm
Shun Li
Shun
Li
University of Ulm
Zhenghui Li
Zhenghui
Li
University of Ulm
David Bayer
David
Bayer
University of Ulm
Alison Conquest
Alison
Conquest
The Alfred Hospital
Anna M. Hagenston
Anna M.
Hagenston
Heidelberg University
Albert Ludolph
Albert
Ludolph
German Center for Neurodegenerative Diseases
University of Ulm
Markus Huber-Lang
Markus
Huber-Lang
University of Ulm
Tobias Boeckers
Tobias
Boeckers
German Center for Neurodegenerative Diseases
University of Ulm
Bernd Knöll
Bernd
Knöll
University of Ulm
Maria Cristina Morganti-Kossmann
Maria Cristina
Morganti-Kossmann
University of Arizona
The Alfred Hospital
Hilmar Bading
Hilmar
Bading
Heidelberg University
Francesco Roselli
Francesco
Roselli
German Center for Neurodegenerative Diseases
University of Ulm
Additional file 2 of Neuronal nuclear calcium signaling suppression of microglial reactivity is mediated by osteoprotegerin after traumatic brain injury
Additional file 2: Figure S2. Blunting neuronal nuclear calcium signaling did not alter IBA1+/TMEM119+ cell density and percentage in the perilesional area post-TBI. A) Depiction of the regions of interest: the core area of the injury and the perilesional areas 200µm away from the injury axis. Scale bar 200µm. B) No significant difference in IBA1+ and IBA1+/CD169+ cell density in the perilesional area 24h post injury (IBA1+: CS= 4.10 ± 0.47, CT = 6.73 ± 1.62 and PT = 8.92 ± 1.84; IBA1+/CD169+ CS = 0.18 ± 0.25, CT = 1.12 ± 1.48 and PT = 1.62 ± 1.44). Significant increase in IBA1+/CD11c+ cell density after TBI but unaltered by nuclear calcium buffering (CS vs CT 0.5 ± 0.39 vs 5.5 ± 1.77; CT vs PT 5.5 ± 1.77 vs 5.5 ± 0.97). IBA1+/CST7+ cell density is significantly increased by nuclear calcium buffering (CT vs PT 1.25 ± 0.3361 vs 3.175 ± 0.4787) Data are shown as mean ± SD. N=4. *p<0.05, **p<0.01; ***: p < 0.001. C) No significant differences in fractions of TMEM119+ and CD169+ cells 24h post injury (TMEM119+: CT vs. PT 95.91 ± 2.5% vs PT = 97.69 ± 0.87%; CD169+: CT vs PT 29.29 ± 41.73% vs 22.68 ± 20.96). Significant increase in CD11+ expression 24h after injury but unaltered by PV.NLS (CS vs CT 12.17 ± 10.2% vs 80.3 ± 8.32%; CT vs PT 80.3 ± 8.32% vs 64.44 ± 20.57%). Significant increase of CST7+ expression 24h after injury after nuclear calcium buffering (CT vs PT 28.19 ± 5.235% vs 50.32 ± 15.8%). Data shown as mean ± SD. N=4. **p<0.01, ***p<0.001. D)-F) Significant decrease of inhibitory (Gephryn) synaptic density 24h post-TBI in the core (CS vs CT; 870.5 ± 99.78 vs 574 ± 70.83) and in the perilesional area (CS vs CT; 1021 ± 161.5 vs 701.6 ± 31.64). Buffering of nuclear calcium signaling blunted the synaptic loss in the core (CT vs PT; 574 ± 70.83 vs 681.8 ± 74.02) but did not alter the synaptic density in the perilesional area (CT vs PT; 701.6 ± 31.64 vs 755.5 ± 85.07). Data are shown as mean ± SD. N = 4. **: p < 0.01; ****: p < 0.0001. Scale bar 100µm (overview) and 5µm (insert).
Medicine
Cell Biology
Genetics
Molecular Biology
Neuroscience
Physiology
Infectious Diseases
figshare
2022
2022-11-22
2024-02-09
Figure
20802616 Bytes
10.6084/m9.figshare.21601415
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