10.6084/M9.FIGSHARE.21541393
Qi Di
Qi
Di
Yi Li
Yi
Li
Danping Zhang
Danping
Zhang
Wei Wu
Wei
Wu
Lin Zhang
Lin
Zhang
Xing Zhao
Xing
Zhao
Li Luo
Li
Luo
Liangliang Yu
Liangliang
Yu
A novel type of phytosulfokine, PSK-ε, positively regulates root elongation and formation of lateral roots and root nodules in <i>Medicago truncatula</i>
<p>Phytosulfokines (PSKs) are a class of tyrosine-sulfated pentapeptides. PSK-α, PSK-γ, and PSK-δ are three reported PSK members involved in regulating plant growth, development, and resistance to biotic and abiotic stresses. Here, we reported a novel type of PSK, PSK-ε with the sequence Y<sub>SO3</sub>VY<sub>SO3</sub>TN, and its precursor proteins (MtPSKε, LjPSKε, and GmPSKε), specifically from legume species. PSK-ε peptide differs from PSK-δ by one amino acid and is close to PSK-δ in the phylogenetic relationship. Expression profile analysis showed that <i>MtPSKε</i> was highly expressed in <i>Medicago truncatula</i> roots, especially in root tips and emerged lateral roots. Application of the synthetic sulfated PSK-ε peptide and overexpression of <i>MtPSKε</i> significantly promoted <i>M. truncatula</i> root elongation and increased lateral root number, probably by inducing cell division and expansion in roots. Furthermore, <i>MtPSKε</i> expression was induced by rhizobia infection and was detected in root nodules including nodule primordia. Both PSK-ε peptide treatment and <i>MtPSKε</i> overexpression significantly increased nodule number in <i>M. truncatula</i>. Taken together, these results demonstrate that PSK-ε, a novel type of phytosulfokine, positively regulates root elongation and formation of lateral root and root nodule in <i>M. truncatula</i>.</p>
Molecular Biology
Ecology
Biological Sciences not elsewhere classified
Developmental Biology
Infectious Diseases
Plant Biology
Taylor & Francis
2022
2022-11-11
2024-03-21
Dataset
2215087 Bytes
10.1080/15592324.2022.2134672
CC BY 4.0