10.6084/M9.FIGSHARE.21405704.V1
Donatella Delle Cave
Donatella Delle
Cave
0000-0002-4019-2778
National Research Council
Silvia Buonaiuto
Silvia
Buonaiuto
National Research Council
Bruno Sainz
Bruno
Sainz
Instituto Ramón y Cajal de Investigación Sanitaria
Alberto Sols Biomedical Research Institute
Centro de Investigación Biomédica en Red de Cáncer
Marco Fantuz
Marco
Fantuz
Venetian Institute of Molecular Medicine
University of Padua
Maria Mangini
Maria
Mangini
National Research Council
Alessandro Carrer
Alessandro
Carrer
Venetian Institute of Molecular Medicine
University of Padua
Annalisa Di Domenico
Annalisa
Di Domenico
National Research Council
Tea Teresa Iavazzo
Tea Teresa
Iavazzo
National Research Council
Gennaro Andolfi
Gennaro
Andolfi
National Research Council
Carme Cortina
Carme
Cortina
Centro de Investigación Biomédica en Red de Cáncer
Institute for Research in Biomedicine
Marta Sevillano
Marta
Sevillano
Centro de Investigación Biomédica en Red de Cáncer
Institute for Research in Biomedicine
Christopher Heeschen
Christopher
Heeschen
Shanghai Jiao Tong University
Vincenza Colonna
Vincenza
Colonna
National Research Council
Marco Corona
Marco
Corona
National Research Council
Antonio Cucciardi
Antonio
Cucciardi
National Research Council
Martina Di Guida
Martina
Di Guida
National Research Council
Eduard Batlle
Eduard
Batlle
Centro de Investigación Biomédica en Red de Cáncer
Institute for Research in Biomedicine
Annachiara De Luca
Annachiara
De Luca
National Research Council
Enza Lonardo
Enza
Lonardo
0000-0001-6654-1043
National Research Council
Additional file 3 of LAMC2 marks a tumor-initiating cell population with an aggressive signature in pancreatic cancer
Additional file 3: Figure S3. LAMC2 expression correlates with CSC content and function. (a) qPCR analysis of LAMC2 gene expression in adherent cells versus spheres. Data are normalized to GAPDH and are presented as fold change in gene expression relative to adherent cells (indicated as Adh). (b) Western blot analysis of LAMC2 in adherent cells versus spheres. Parallel β-ACTIN immunoblotting was performed. (c) qPCR analysis for CD44, CD133 and L1CAM genes in adherent cells versus spheres. Data are normalized to GAPDH and are presented as fold change in gene expression relative to adherent cells. (d) Correlation between CD44 and LAMC2 in PDAC samples from the TCGA dataset. The p value is based on Pearson’s Correlation. (e) qPCR analysis for LAMC2 in PDAC cells grown in different culture conditions. (f) qPCR analysis for LAMC2, ABCG2, CD133, L1CAM and SOX2 in Adh vs Spheres vs Differentiated cells. Data are normalized to GAPDH and are presented as fold change in gene expression relative to adherent cells. (g) Representative images of L3.6pl and PANC-1 cells growth after short (24 hours) or long (7 days) times in Diff. medium compared to CSC medium. (h) qPCR analysis for CD44 and LAMC2 genes in CD44+ sorted cells. Data are normalized to GAPDH and are presented as fold change in gene expression relative to CD44-cells. (i) qPCR analysis for CD133 and LAMC2 genes in CD133+ sorted cells. Data are normalized to GAPDH and are presented as fold change in gene expression relative to CD133- cells. (j) qPCR analysis for LAMC2 in PDAC cells treated with 100 µM of gemcitabine. Data are normalized to GAPDH and are presented as fold change in gene expression relative to control cells (untreated). *p<0.05, **p<0.005, ***p<0.0005. n $$\ge 3.$$ ≥ 3 . Statistical significance was assessed by Student's t-test.
Biochemistry
Cell Biology
Genetics
Molecular Biology
Pharmacology
Chemical Sciences not elsewhere classified
Immunology
Biological Sciences not elsewhere classified
Developmental Biology
Cancer
figshare
2022
2022-10-27
2024-02-26
Journal contribution
6778548 Bytes
10.6084/m9.figshare.21405704
10.1186/s13046-022-02516-w
CC BY + CC0