10.6084/M9.FIGSHARE.21405692.V1
Donatella Delle Cave
Donatella Delle
Cave
0000-0002-4019-2778
National Research Council
Silvia Buonaiuto
Silvia
Buonaiuto
National Research Council
Bruno Sainz
Bruno
Sainz
Instituto Ramón y Cajal de Investigación Sanitaria
Alberto Sols Biomedical Research Institute
Centro de Investigación Biomédica en Red de Cáncer
Marco Fantuz
Marco
Fantuz
Venetian Institute of Molecular Medicine
University of Padua
Maria Mangini
Maria
Mangini
National Research Council
Alessandro Carrer
Alessandro
Carrer
Venetian Institute of Molecular Medicine
University of Padua
Annalisa Di Domenico
Annalisa
Di Domenico
National Research Council
Tea Teresa Iavazzo
Tea Teresa
Iavazzo
National Research Council
Gennaro Andolfi
Gennaro
Andolfi
National Research Council
Carme Cortina
Carme
Cortina
Centro de Investigación Biomédica en Red de Cáncer
Institute for Research in Biomedicine
Marta Sevillano
Marta
Sevillano
Centro de Investigación Biomédica en Red de Cáncer
Institute for Research in Biomedicine
Christopher Heeschen
Christopher
Heeschen
Shanghai Jiao Tong University
Vincenza Colonna
Vincenza
Colonna
National Research Council
Marco Corona
Marco
Corona
National Research Council
Antonio Cucciardi
Antonio
Cucciardi
National Research Council
Martina Di Guida
Martina
Di Guida
National Research Council
Eduard Batlle
Eduard
Batlle
Centro de Investigación Biomédica en Red de Cáncer
Institute for Research in Biomedicine
Annachiara De Luca
Annachiara
De Luca
National Research Council
Enza Lonardo
Enza
Lonardo
0000-0001-6654-1043
National Research Council
Additional file 10 of LAMC2 marks a tumor-initiating cell population with an aggressive signature in pancreatic cancer
Additional file 10: Figure S10. Inhibition of transforming growth factor beta (TGF-β) signaling blocks LAMC2-induced metastasis. (a) Enrichment plots for pancreatic cancer, focal adhesion, hypoxia, MAPK signaling, glycolysis and gluconeogenesis pathways in EGFP+ versus EGFP- cells isolated by FACS from subcutaneous tumors. (b) Western blot analysis for pSMAD2 and SMAD2 in EGFP- versus EGFP+ PANC-1 and #253 cells. Parallel GAPDH immunoblotting was performed. (c) Flow cytometry quantification of EGFP and ALK5-TGFβR1 in EGFP- and EGFP+ L3.6pl and PANC-1 cells. (d) ) Flow cytometry quantification of EGFP and CD44 in EGFP- and EGFP+ L3.6pl and PANC-1 cells treated with 10 ng/ml of recombinant TGF-β1 (rTGF-β1). (e) Representative immunofluorescence images of LAMC2 (red) and nuclei (blue, DAPI) in PDAC cells treated with 10 ng/ml of recombinant TGF-β (rTGF-β1 and rTGF-β2). (f) Correlation between TGFβR1, TGFβR2, TGF-β1, TGF-β2, SMAD2, SMAD4 and LAMC2 in PDAC samples from the TCGA dataset. The p value is based on Pearson’s Correlation. (g) Quantification of lung metastasis area in H&E-stained sections. *p<0.05, n $$\ge 5.$$ ≥ 5 . Statistical significance was assessed by Student's t-test.
Biochemistry
Cell Biology
Genetics
Molecular Biology
Pharmacology
Chemical Sciences not elsewhere classified
Immunology
Biological Sciences not elsewhere classified
Developmental Biology
Cancer
figshare
2022
2022-10-27
2024-02-26
Journal contribution
7755920 Bytes
10.6084/m9.figshare.21405692
10.1186/s13046-022-02516-w
CC BY + CC0