10.6084/M9.FIGSHARE.21341610.V1
Gerrit A. Martens
Gerrit A.
Martens
Universität Hamburg
Cornelia Geßner
Cornelia
Geßner
Universität Hamburg
Carina Osterhof
Carina
Osterhof
Johannes Gutenberg University of Mainz
Thomas Hankeln
Thomas
Hankeln
Johannes Gutenberg University of Mainz
Thorsten Burmester
Thorsten
Burmester
Universität Hamburg
Additional file 1 of Transcriptomes of Clusterin- and S100B-transfected neuronal cells elucidate protective mechanisms against hypoxia and oxidative stress in the hooded seal (Cystophora cristata) brain
Additional file 1: Figure S1. Expression of endogenic and transgenic CLU and S100B sequences in transfected HN33 cell lines at normoxia, determined by qPCR experiments. Differences in Ct-values between endogenic and transgenic nCLU, sCLU and S100B were 13.17 (with Ct of 40 for endogenic nCLU), 13.25 and 9.48, respectively. The fold-expression difference for nCLU, sCLU and S100B therefore were 213.17, 213.25 and 29.48, respectively. Figure S2. TPM values of endogenic (CLU, S100B) and transgenic [CLU (Ccr), S100B (Ccr)] sequences in transfected cell lines (mock, nCLU, sCLU, S100B) at normoxia, hypoxia and oxidative stress. Table S1. Sequencing and mapping overview. Triplicates were sequenced per cell line and oxygen treatment. For replicate mock-H2O2-2 sequencing failed and was discarded. Around 51 million reads per sample were generated of which around 75% mapped to the GRCm39 mouse reference genome.
Genetics
figshare
2022
2022-10-16
2022-10-16
Journal contribution
144080 Bytes
10.1186/s12868-022-00744-6
10.6084/m9.figshare.21341610
CC BY + CC0