10.6084/M9.FIGSHARE.21268964
Yinghang Liu
Yinghang
Liu
Shandong University
Jin Zhang
Jin
Zhang
Shandong University
Qingbin Li
Qingbin
Li
Shandong University
Zhaoxuan Wang
Zhaoxuan
Wang
Shandong University
Zhiyong Cui
Zhiyong
Cui
Shandong University
Tianyuan Su
Tianyuan
Su
Shandong University
Xuemei Lu
Xuemei
Lu
Shandong University
Qingsheng Qi
Qingsheng
Qi
Shandong University
Jin Hou
Jin
Hou
Shandong University
Additional file 1 of Engineering Yarrowia lipolytica for the sustainable production of β-farnesene from waste oil feedstock
Additional file 1: Figure S1. Screening β-farnesene synthase from different plants. A The evolutionary tree of β-farnesene synthase from different plants. B β-Farnesene production and biomass of strains containing different β-farnesene synthase. Data represent the mean ± SD of biological triplicate. Figure S2. The SDS-PAGE electrophoresis analysis of purified protein AanFSK197T/F180H and AanFS. Figure S3. The copy number and relative expression of AanFSK197T/F180H in Q6 and Q7 strains. Data represent the mean ± SD of biological duplicate. Figure S4. Intracellular lipids accumulation in PO1f, CP7 and Q12 strain. Data represent the mean ± SD of biological triplicate. Figure S5. Fermentation conditions optimization of Q26 strain in 5 L bioreactor. A β-Farnesene production at different air flux and stirring rate. B Growth curve at different air flux and stirring rate during fermentation. C β-Farnesene production at different pH. D Growth curve at different pH. β-Farnesene detected after 96 h of fermentation. Data represent the mean ± SD of biological duplicate. Figure S6. The relationship of the engineered strains used in this study. CP7 strain was obtained by locating the synthesis pathway of mevalonate in the peroxisome of AHH12 strain. Different genes of MVA pathway were successively overexpressed in CP7 strain to obtain Q3–Q7 strains, respectively. On the basis of strain Q7, the genes involved in β-oxidation were overexpressed, respectively, to construct strain Q8–Q14, and Q17–Q21 strain was obtained by overexpressing other genes related to β-oxidation basing on Q12 strain. Q15 strain was constructed by regulating citric acid metabolism of Q7 strain and Q16 strain was derived from Q15 strain. Expressing citric acid regulating genes in Q12 strain obtained Q22 strain. On the basis of strain Q12, the fatty acid anabolism was regulated to construct Q23–Q26 strain. On the basis of strain Q26, the combinatorial regulation of fatty acid anabolism obtained Q27 strain. Table S1. The primary primers used in this study. Table S2. The codon optimized sequences of β-farnesene synthase from different plants. Table S3. The detailed information of genes used in this study. Table S4. The accumulation of by-products at 216 h of fermentation. Data represent the mean ± SD of biological duplicate.
Biochemistry
Space Science
Microbiology
Molecular Biology
Pharmacology
Biotechnology
Chemical Sciences not elsewhere classified
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Inorganic Chemistry
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2022
2022-10-04
2023-07-02
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