10.6084/M9.FIGSHARE.20920161.V1
Wei Sun
Wei
Sun
Shanshan Liu
Shanshan
Liu
Anfa Lu
Anfa
Lu
Fan Yang
Fan
Yang
Junhong Duan
Junhong
Duan
<i>In vitro</i> anti-PRV activity of dihydromyricetin from <i>Ampelopsis grossedentata</i>
<p>Pseudorabies (PR) is an acute infectious disease caused by pseudorabies virus (PRV). There are no available drugs due to the emergence of variant of PRV. Dihydromyricetin (DMY) is a flavonoid extracted from <i>Ampelopsis grossedentata</i> (<i>A. grossedentata</i>), which has a variety of pharmacological activities. In this study, we aim to investigate the <i>in vitro</i> anti-PRV activity of DMY extracted from <i>A. grossedentata</i>. MTT assay was used to detect the cytotoxicity and antiviral activity of DMY. The results detected by flow cytometry and qRT-PCR showed that DMY played anti-PRV role mainly by interfering with the process of virus invasion into host cell and inhibiting the occurrence of pyroptosis <i>in vitro.</i> This suggested that anti-pyroptosis may be an important antiviral mechanism for DMY which is expected to be a potential anti-PRV drug.</p>
Biochemistry
Microbiology
Molecular Biology
Pharmacology
Biotechnology
Environmental Sciences not elsewhere classified
Biological Sciences not elsewhere classified
Cancer
Science Policy
Virology
Taylor & Francis
2022
2022-09-05
2024-02-29
Journal contribution
1506743 Bytes
10.6084/m9.figshare.20920161
10.1080/14786419.2021.1982935
CC BY 4.0