10.6084/M9.FIGSHARE.20453999.V1
Xiaoyu Wang
Xiaoyu
Wang
Chengcheng Liang
Chengcheng
Liang
Anning Li
Anning
Li
Gong Cheng
Gong
Cheng
Feng Long
Feng
Long
Rajwali Khan
Rajwali
Khan
Jianfang Wang
Jianfang
Wang
Yu Zhang
Yu
Zhang
Sen Wu
Sen
Wu
Yujuan Wang
Yujuan
Wang
Ju Qiu
Ju
Qiu
Chugang Mei
Chugang
Mei
Wucai Yang
Wucai
Yang
Linsen Zan
Linsen
Zan
RNA-Seq and lipidomics reveal different adipogenic processes between bovine perirenal and intramuscular adipocytes
<p>Adipogenesis involves complex interactions between transcription and metabolic signalling. Exploration of the developmental characteristics of intramuscular adipocyte will provide targets for enhancing beef cattle marbling without increasing obesity. Few reports have compared bovine perirenal and intramuscular adipocyte transcriptomes using the combined analysis of transcriptomes and lipid metabolism to explore differences in adipogenic characteristics. We identified perirenal preadipocytes (PRA) and intramuscular preadipocytes (IMA) in Qinchuan cattle. We found that IMA were highly prolific in the early stages of adipogenesis, while PRA shows a stronger adipogenic ability in the terminal differentiation. Bovine perirenal and intramuscular adipocytes were detected through the combined analysis of the transcriptome and metabolome. More triglyceride was found to be upregulated in perirenal adipocytes; however, more types and amounts of unsaturated fatty acids were detected in intramuscular adipocytes, including eicosapentaenoic acid (20:5 n-3; EPA) and docosahexaenoic acid (22:6 n-3; DHA). Furthermore, differentially expressed genes in perirenal and intramuscular adipocytes were positively correlated with the eicosanoid, phosphatidylcholine (PC), phosphatidyl ethanolamine (PE), and sphingomyelin contents. Associated differential metabolic pathways included the glycerolipid and glycerophospholipid metabolisms. Our research findings provide a basis for the screening of key metabolic pathways or genes and metabolites involved in intramuscular fat production in cattle.</p>
Biochemistry
Cell Biology
Genetics
Physiology
Chemical Sciences not elsewhere classified
Biological Sciences not elsewhere classified
Virology
Computational Biology
Taylor & Francis
2022
2022-08-09
2024-02-05
Dataset
68304784 Bytes
10.6084/m9.figshare.20453999
10.1080/21623945.2022.2106051
CC BY 4.0