10.6084/M9.FIGSHARE.20071817.V1
Jun Teng
Jun
Teng
Dan Wang
Dan
Wang
Chinese University of Hong Kong
Zhejiang University
University Medical Center Hamburg-Eppendorf
Shanghai Jiao Tong University
University of Toronto
Chongqing Medical University
Central South University
Nankai University
China Agricultural University
Hebei Normal University
Hubei University of Chinese Medicine
Hunan Agricultural University
Nantong University
University of Ontario Institute of Technology
Southern Medical University
Chinese Academy of Agricultural Sciences
Guangzhou Medical University
China West Normal University
China Medical University
Second Affiliated Hospital of Chongqing Medical University
Shanghai University of Traditional Chinese Medicine
Shuguang Hospital
First Affiliated Hospital of Zhengzhou University
Affiliated Hospital of Xuzhou Medical College
Henan Provincial People's Hospital
First Affiliated Hospital of Wenzhou Medical University
Beijing Proteome Research Center
Xi'an Jiaotong University
Shaanxi Provincial People's Hospital
Shandong Agricultural University
Hubei University for Nationalities
Guangzhou Sport University
Institute of Plant Protection
Liaoning Technical University
University of Science and Technology of China
Sichuan Agricultural University
Wenwen Wang
Wenwen
Wang
Air Force Medical University
Xuzhou Medical College
Chao Ning
Chao
Ning
China Agricultural University
Shandong Agricultural University
Max Planck Institute for the Science of Human History
Qin Zhang
Qin
Zhang
University of Oulu
Zhejiang University
Shanghai Jiao Tong University
Shanxi Medical University
Soochow University
Zhejiang Chinese Medical University
Huazhong University of Science and Technology
Army Medical University
Anhui Agricultural University
China Medical University
First Affiliated Hospital of Nanchang University
First Affiliated Hospital of Anhui Medical University
West China Hospital of Sichuan University
Second Affiliated Hospital of Kunming Medical College
Zhejiang Provincial People's Hospital
Shandong Agricultural University
Jiangsu Cancer Hospital
Second Affiliated Hospital of Xi'an Jiaotong University
Simcere Pharmaceutical (China)
Hui Tang
Hui
Tang
Chinese Academy of Medical Sciences & Peking Union Medical College
Nanjing Medical University
Transcriptional Regulation of the Chicken <i>ASMT</i> Gene- A Preliminary Analysis
<p>1. Melatonin is an indole hormone that, among its myriad biological functions, regulates circadian and seasonal rhythms in animals. The <i>ASMT</i> gene plays an essential role in melatonin synthesis. However, in chickens, little is known about the regulatory elements governing its transcription.</p> <p>2. The following study identified the transcription start site of the chicken <i>ASMT</i> gene by 5<i>’</i>-RACE. Then, the proximal minimal promoter was identified using a series of 5′ truncations of the <i>ASMT</i> promoter (<i>e.g</i>., -3502/+17, -2698/+17, -2003/+17, -1378/+17, and -254/+17). Site-directed mutagenesis, overexpression, and electrophoretic mobility shift assay (EMSA) were applied to show that the transcription factor Oct-1 binds to the promoter region of <i>ASMT</i>.</p> <p>3. The translation start site was located 19 bp upstream from the translational start site. The luciferase reporter assay confirmed that the core promoter of chicken <i>ASMT</i> gene was in the -254/+17 region. Using site-directed mutagenesis, overexpression, and EMSA, Oct-1 bound the promoter of <i>ASMT</i>.</p> <p>4. Overall, Oct1 plays an important role in the transcriptional regulation of chicken <i>ASMT</i> gene.</p>
Cell Biology
Genetics
Molecular Biology
59999 Environmental Sciences not elsewhere classified
39999 Chemical Sciences not elsewhere classified
Developmental Biology
Cancer
Hematology
Computational Biology
Taylor & Francis
2022
2022-06-15
2023-01-06
Online resource
0 Bytes
10.1080/00071668.2022.2090230
10.6084/m9.figshare.20071817
CC BY 4.0