10.6084/M9.FIGSHARE.19941215
Hong-Shi Li
Hong-Shi
Li
Jiang-Ying Kuang
Jiang-Ying
Kuang
Gui-Jun Liu
Gui-Jun
Liu
Wei-Jie Wu
Wei-Jie
Wu
Xian-Lun Yin
Xian-Lun
Yin
Hao-Dong Li
Hao-Dong
Li
Lei Wang
Lei
Wang
Tao Qin
Tao
Qin
Wen-Cheng Zhang
Wen-Cheng
Zhang
Yuan-Yuan Sun
Yuan-Yuan
Sun
Myostain is involved in ginsenoside Rb1-mediated anti-obesity
<p>Obesity, one of the major public health problems worldwide, has attracted increasing attention. Ginsenoside Rb1 is the most abundant active component of <i>Panax ginseng</i> C.A.Mey (Araliaceae) and is reported to have beneficial effects on obesity and diabetes. However, the mechanisms by which Rb1 regulates obesity remain to be explored.</p> <p>This paper intends to further explore the mechanism of Rb1 in regulating obesity.</p> <p>The C57BL/6 obese mice were divided into two groups: the control (CTR) and Rb1. The CTR group [intraperitoneally (ip) administered with saline] and the Rb1 group (ip administered with Rb1, 40 mg/kg/d) were treated daily for four weeks. <i>In vitro</i>, Rb1 (0, 10, 20, 40 μM) was added to differentiated C2C12 cells and Rb1 (0, 20, 40 μM) was added to 3T3-L1 cells. After 24 h, total RNA and protein from C2C12 cells and 3T3-L1 cells were used to detect myostatin (MSTN) and fibronectin type III domain-containing 5 (FNDC5) expression.</p> <p>Rb1 reduced the body weight and adipocyte size. Improved glucose tolerance and increased basic metabolic activity were also found in Rb1 treated mice. MSTN was downregulated in differentiated C2C12 cells, 3T3-L1 cells and adipose tissues upon Rb1 treatment. FNDC5 was increased after Rb1 treatment. However, MSTN overexpression attenuated Rb1-mediated decrease accumulation of lipid droplets in differentiated 3T3-L1 adipocytes.</p> <p>Rb1 may ameliorate obesity in part through the MSTN/FNDC5 signalling pathway. Our results showed that Rb1 can be used as an effective drug in the treatment of human obesity.</p>
Biochemistry
Cell Biology
Genetics
Molecular Biology
Physiology
Environmental Sciences not elsewhere classified
Chemical Sciences not elsewhere classified
Biological Sciences not elsewhere classified
Developmental Biology
Cancer
Computational Biology
Taylor & Francis
2023
2023-01-06
2024-03-25
Dataset
1325821 Bytes
10.1080/13880209.2022.2074056
CC BY 4.0