10.6084/M9.FIGSHARE.19624404
Xuyang Song
Xuyang
Song
Meng Zhao
Meng
Zhao
Qiaoqiao Cao
Qiaoqiao
Cao
Shengxuan Wang
Shengxuan
Wang
Ranran Li
Ranran
Li
Xuan Zhang
Xuan
Zhang
Letian Zhang
Letian
Zhang
Kerong Shi
Kerong
Shi
Transcriptome provides insights into bovine mammary regulatory mechanisms during the lactation cycle
<p>The molecular events underlying the lactation curve of dairy animals have long been a biological conundrum for mammary biologists, and a cause of a considerable loss of income for dairy farmers. To better understand the regulatory mechanisms and developmental processes of the mammary gland during the lactation cycle, whole-genome transcriptome profiles of bovine mammary tissues at five different lactation stages were investigated using the RNA-Seq technique. The differentially expressed genes were identified by comparing different lactation stage combinations. Gene expression changes were validated using quantitative reverse-transcription polymerase chain reaction. Some differentially expressed genes were found to locate in the known quantitative trait loci regions that are associated with multiple milk production traits. Proteins of potentially important genes were confirmed to be expressed in the mammary epithelium of dairy cows. An integrated analysis of differential gene expression indicated that internal development regularities underlying the shape of the milk production curve. First, the mammary gland is enriched in similar activities during lactation onset and involution, but in opposite regulatory directions. Second, the lactation stages before and after the milk peak shared similar biological processes that were mainly involved in extracellular matrix remodelling. The discoveries of the study provide information necessary to improve milk production.</p>
Medicine
Cell Biology
Genetics
Molecular Biology
Physiology
Environmental Sciences not elsewhere classified
Immunology
Biological Sciences not elsewhere classified
Developmental Biology
Cancer
Plant Biology
Computational Biology
Taylor & Francis
2022
2022-04-21
2023-06-01
Dataset
3039841 Bytes
10.1080/09712119.2022.2064865
CC BY 4.0