10.6084/M9.FIGSHARE.19605940.V1
Weijing Yao
Weijing
Yao
Yixing Li
Yixing
Li
Yingcong Chen
Yingcong
Chen
Yuting Chen
Yuting
Chen
Yu Xie
Yu
Xie
Miaojuan Ye
Miaojuan
Ye
Ying Zhang
Ying
Zhang
Xiangjun Chen
Xiangjun
Chen
Xiaoyong Wu
Xiaoyong
Wu
Yuyao Feng
Yuyao
Feng
Zhi Hong
Zhi
Hong
Yigang Wang
Yigang
Wang
Wei Liu
Wei
Liu
Cong Yi
Cong
Yi
Atg1-mediated Atg11 phosphorylation is required for selective autophagy by regulating its association with receptor proteins
<p>Atg11 is an adaptor protein required for the induction of selective autophagy via receptor binding. However, our understanding of the molecular mechanisms by which it regulates selective autophagy remains incomplete. Here, we show that Atg11 is phosphorylated by Atg1. Rapamycin treatment or starvation conditions induced slower electrophoretic mobility of Atg11 in an Atg1 kinase activity-dependent manner. Through <i>in vitro</i> kinase assays combined with mutagenesis, we determined that Atg1 phosphorylates S949, S1057, and S1064 residues in CC4 domain of Atg11. Replacing the three residues with alanine suppressed the cleavage of selective autophagy substrates for the cytoplasm-to-vacuole targeting (Cvt) pathway, mitophagy, reticulophagy, and pexophagy. The Atg11 mutant was defective in binding to related selective autophagy receptors. These results demonstrate a previously unknown function of Atg1 in regulation of selective autophagy via Atg11 phosphorylation.</p> <p><b>Abbreviations</b>: AMPK: AMP-activated protein kinase; ATG: autophagy-related; Cvt: cytoplasm-to-vacuole targeting; FUNDC1: FUN14 domain-containing protein 1; GFP: green fluorescent protein; MTOR: mechanistic target of rapamycin kinase; PAS: phagophore assembly site; PIK3C3: phosphatidylinositol 3-kinase catalytic subunit type 3; PRKAC/PKA: protein kinase cAMP-activated; SD-G: glucose starvation; SD-N: nitrogen starvation; ULK1: unc-51 like autophagy activating kinase 1; λ-PPase: lambda protein phosphatase.</p>
Biochemistry
Cell Biology
Genetics
Molecular Biology
Immunology
Cancer
Inorganic Chemistry
Plant Biology
Taylor & Francis
2022
2022-04-15
2024-02-13
Journal contribution
798033 Bytes
10.6084/m9.figshare.19605940
10.1080/15548627.2022.2063494
CC BY 4.0