10.6084/M9.FIGSHARE.19586561
Yujia Bai
Yujia
Bai
Bibo Ye
Bibo
Ye
Tianyu Li
Tianyu
Li
Rongrong Wang
Rongrong
Wang
Xiaofei Qi
Xiaofei
Qi
H22954, a long non-coding RNA, inhibits glucose uptake in leukemia cells in a GLUT10-dependent manner
<p>To investigate the performance of H22954, a novel long non-coding RNA (lncRNA), in inhibiting glucose uptake in leukemia cells.</p> <p><sup>18</sup>F-FDG uptake, RNA half-life quantitative real-time polymerase chain reaction (qRT-PCR) and luciferase assays were performed to detect the glucose uptake in the condition of leukemia. Microarrays and qRT-PCR analyses were used to identify the related genes or proteins and elucidate the underlying these processes.</p> <p>H22954, a novel lncRNA, inhibited glucose uptake in leukemia cells. Using bioinformatics and microarray analyses, GLUT10 was identified as a possible target molecule of H22954. H22954 targeted the 3′untranslated region of GLUT10. In the luciferase assay, the luciferase activity of pGL3-GLUT10 was inhibited by H22954. Consistently, H22954 expression levels were inversely correlated with GLUT10 expression in cell lines and acute myeloid leukemia (AML) samples. Conversely, the degradation rate of <i>GLUT10</i> mRNA was increased after H22954 overexpression. Moreover, glucose uptake was recovered when the GLUT10-interaction sites in H22954 were mutated.</p> <p>The lncRNA H22954 regulated GLUT10 expression to inhibit glucose uptake in leukemia cells. Our findings provide potentially valuable data for designing new targeted strategies based on H22954.</p>
Biochemistry
Cell Biology
Genetics
Molecular Biology
Physiology
Pharmacology
Chemical Sciences not elsewhere classified
Biological Sciences not elsewhere classified
Cancer
Hematology
Virology
Taylor & Francis
2022
2022-04-13
2023-06-02
Dataset
166850 Bytes
10.1080/16078454.2022.2061112
CC BY 4.0