10.6084/M9.FIGSHARE.19382158.V1
Yongqi Yue
Yongqi
Yue
Yonglin Hua
Yonglin
Hua
Jing Zhang
Jing
Zhang
Yu Guo
Yu
Guo
Dan Zhao
Dan
Zhao
Wentao Huo
Wentao
Huo
Yan Xiong
Yan
Xiong
Fenfen Chen
Fenfen
Chen
Yaqiu Lin
Yaqiu
Lin
Xianrong Xiong
Xianrong
Xiong
Jian Li
Jian
Li
Establishment of a subcutaneous adipogenesis model and distinct roles of LKB1 regulation on adipocyte lipid accumulation in high-altitude <i>Bos grunniens</i>
<p>The domestic yak was regarded as a valuable model to explore the molecular mechanism of high-altitude animal adaptation, including the extreme tolerance of starvation and cold stress, which is closely associated with specifically seasonal deposition and metabolism of subcutaneous adipose tissue. However, the key regulators for these processes remain largely unknown. Here, the yak adipogenesis model in vitro was established and liver kinase b1 (<i>LKB1</i>) was regarded as a key negative regulator involved in yak adipocytes lipid accumulation. First, the mRNA level of <i>LKB1</i> in adipose tissue of yaks from the cold season is significantly higher than that of yaks from the warm season. Interestingly, overexpression of <i>LKB1</i> significantly inhibited adipocytes lipid accumulation. Consistently, <i>LKB1</i> overexpression dramatically suppressed mRNA levels of adipogenic transcriptional factors, including <i>C/EBPα</i>, <i>C/EBPβ</i> and <i>PPARγ</i>, and subsequently significantly decreased levels of lipid accumulation related genes (<i>FABP4</i> and <i>FAS</i>) compared with those of control. In contrast, overexpression of <i>LKB1</i> promoted thermogenic and lipolysis related genes in yak adipocyte, including <i>PGC1α</i>, <i>PPARα</i>, <i>UCP1</i>, <i>ATGL</i>, <i>HSL</i> and <i>LPL</i>. Therefore, these results would provide new insights to decipher the network for domestic yak adipose deposition, and <i>LKB1</i> might be as a new molecular target for animal environmental-resistant breeding.</p>
Biochemistry
Cell Biology
Genetics
Molecular Biology
Physiology
Environmental Sciences not elsewhere classified
Chemical Sciences not elsewhere classified
Biological Sciences not elsewhere classified
Virology
Computational Biology
Taylor & Francis
2022
2022-03-18
2024-03-01
Dataset
7765688 Bytes
10.6084/m9.figshare.19382158
10.1080/09712119.2022.2042001
CC BY 4.0