10.6084/M9.FIGSHARE.19346232
Joy-Yan Lam
Joy-Yan
Lam
Yau-Yee Ng
Yau-Yee
Ng
Chun-Kit Yuen
Chun-Kit
Yuen
Wan-Man Wong
Wan-Man
Wong
Kwok-Yung Yuen
Kwok-Yung
Yuen
Kin-Hang Kok
Kin-Hang
Kok
A nasal omicron vaccine booster elicits potent neutralizing antibody response against emerging SARS-CoV-2 variants
<p>SARS-CoV-2 has caused the COVID-19 pandemic since early 2020. As of January 2022, the worldwide spreading of SARS-CoV-2 leads to approximately 0.35 billion of human infections and five millions of deaths. Current vaccination is one of the effective ways to control SARS-CoV-2 transmission and reduce the disease severity. However, the antibody level against the immunogen significantly drops several months after the standard two-dose vaccination, and hence a third or fourth dose booster (the same immunogen) has been suggested to boost the antibody response. Here, we described an ultra-effective nasal vaccine booster that potently induced the extraordinary high-level of neutralizing antibody in pre-vaccinated mice. The vaccine booster is composed of a recombinant receptor binding domain of SARS-CoV-2 spike (either wild-type or omicron) fused with a domain of SARS-CoV-2 nucleoprotein. In the absence of adjuvants, a single intranasal administration of the booster in pre-vaccinated mice significantly induced systemic and mucosal antibody responses as evidenced by the elevation of the cross-variant neutralizing antibody and induction of IgA in bronchoalveolar lavage respectively. Most importantly, the single dose nasal vaccine booster (omicron version) potently enhanced the neutralizing activity against authentic SARS-CoV-2 omicron virus infection. Taken together, the induction of respiratory mucosal immunity and the enhancement of cross-variant neutralizing activity by the nasal vaccine booster warrants further clinical trials in humans.</p>
Medicine
Microbiology
Cell Biology
Molecular Biology
Neuroscience
Biotechnology
Immunology
Cancer
Infectious Diseases
Virology
Taylor & Francis
2022
2022-03-30
2023-10-02
Dataset
333235 Bytes
10.1080/22221751.2022.2053365
CC BY 4.0