10.6084/M9.FIGSHARE.18865960.V2
Weijie Min
Weijie
Min
Yina Wu
Yina
Wu
Yibin Fang
Yibin
Fang
Bo Hong
Bo
Hong
Dongwei Dai
Dongwei
Dai
Yu Zhou
Yu
Zhou
Jianmin Liu
Jianmin
Liu
Qiang Li
Qiang
Li
Bone marrow mesenchymal stem cells-derived exosomal microRNA-124-3p attenuates hypoxic-ischemic brain damage through depressing tumor necrosis factor receptor associated factor 6 in newborn rats
<p>Mesenchymal stem cells (MSCs)-derived exosomes (Exo) are beneficial in the use of brain damages. Restrictively, the mechanism of Exo expressing miR-124-3p in hypoxic-ischemic brain damage (HIBD) is not completely comprehended. Thereupon, this work was put forward to reveal the action of bone marrow MSCs-derived Exo (BMSCs-Exo) expressing miR-124-3p in the illness. BMSCs were isolated and transfected with miR-124-3p agomir. Then, BMSCs-Exo were extracted and identified. The newborn HIBD rats were injected with miR-124-3p-modified BMSCs-Exo or tumor necrosis factor receptor associated factor 6 (TRAF6)-related vectors. Next, neurological functions, neuron pathological and structural damages, oxidative stress and neuronal apoptosis were observed. miR-124-3p and TRAF6 expression was tested, along with their targeting relationship. miR-124-3p was down-regulated, and TRAF6 was up-regulated in newborn HIBD rats. miR-124-3p targeted TRAF6. BMSCs-Exo improved neurological functions, alleviated neuron pathological and structural damages, suppressed oxidative stress and reduced neuronal apoptosis in newborn HIBD rats, whereas BMSCs-Exo-mediated effects were enhanced by restoring miR-124-3p. Silencing TRAF6 attenuated HIBD in newborn rats, but overexpression of TRAF6 reversed the protective role of miR-124-3p-overexpressing BMSCs-Exo. This work makes it comprehensive that up-regulated exosomal miR-124-3p ameliorates HIBD in newborn rats by targeting TRAF6, which replenishes the potential agents for curing HIBD.</p>
Biophysics
Biochemistry
Cell Biology
Genetics
Molecular Biology
Neuroscience
Physiology
Immunology
Biological Sciences not elsewhere classified
Developmental Biology
Marine Biology
Science Policy
Taylor & Francis
2022
2022-01-31
2024-03-21
Journal contribution
13494 Bytes
10.6084/m9.figshare.18865960
10.1080/21655979.2021.2016094
CC BY 4.0