10.6084/M9.FIGSHARE.17521493.V1
Mei Ye
Mei
Ye
Xin Chen
Xin
Chen
Lin Duo
Lin
Duo
Jin Ma
Jin
Ma
Le Cao
Le
Cao
Chiyu Zhang
Chiyu
Zhang
Yong-Tang Zheng
Yong-Tang
Zheng
Identification of two novel HIV-1 circulating recombinant forms of CRF111_01C and CRF116_0108 in southwestern Yunnan, China
<p>Yunnan, the region hardest hit by HIV/AIDS in China, is also an area with the most abundant HIV-1 genetic diversity. A large number of novel HIV-1 circulating recombinant forms (CRFs) and unique recombinants were identified among injection drug users in Yunnan; however, few were found among sexual contacts. Here, we obtained 15 near full-length genome sequences (NFLGs) from HIV-1 seropositive heterosexual contacts in Yunnan who received antiretroviral therapy during the period from 2014 to 2016. Phylogenetic analysis showed that six NFLGs belonged to CRF01_AE (n = 3) and CRF106_cpx (n = 3), and the other nine sequences were novel inter-subtype recombinants. Of the recombinants, two novel CRFs (CRF111_01 C (n = 4) and CRF116_0108 (n = 4)) and one CRF106_cpx variant (n = 1) were identified. CRF111_01 C had a CRF01_AE backbone with seven subtype C fragments inserted into the <i>gag, pol, vif, env, nef</i> and <i>3ʹLTR</i> regions. CRF116_0108 had a CRF08_BC backbone with a CRF01_AE fragment inserted into the <i>pol, tat, rev, vif, vpr, vpu</i> and <i>env</i> regions. Phylogeographic analyses estimated that CRF111_01 C and CRF116_0108 originated approximately 1995.7–1998.6 and 1991.7–1993.7, respectively. These identifications of two novel HIV-1 CRFs highlighted the importance of continuous surveillance in heterosexual contacts and other high-risk groups in this region and the surrounding regions.</p>
Biophysics
Biochemistry
Medicine
Microbiology
Cell Biology
Environmental Sciences not elsewhere classified
Sociology
Biological Sciences not elsewhere classified
Cancer
Infectious Diseases
Virology
Taylor & Francis
2021
2021-12-24
2024-03-21
Journal contribution
2520582 Bytes
10.6084/m9.figshare.17521493
10.1080/21505594.2021.2010399
CC BY 4.0