10.6084/M9.FIGSHARE.17092355.V1
Yuyan Liao
Yuyan
Liao
Chenghao Kuang
Chenghao
Kuang
Zheng Bao
Zheng
Bao
Yijing He
Yijing
He
Long Gu
Long
Gu
Qianke Tao
Qianke
Tao
Xiancheng Qiu
Xiancheng
Qiu
Ghosh Dipritu
Ghosh
Dipritu
Xi Kong
Xi
Kong
Lifang Zhang
Lifang
Zhang
Jianhua Peng
Jianhua
Peng
Yong Jiang
Yong
Jiang
Shigang Yin
Shigang
Yin
Nucleo-cytoplasmic RNA distribution responsible for maintaining neuroinflammatory microenvironment
<p>Subcellular localization of transcripts is highly associated with regulation of gene expression, synthesis of protein, and also the development of the human brain cortex. Although many mechanisms are prevalent in the occurrence of neuroinflammation, the mechanisms based on differences in subcellular localization of transcripts have not been explored. To characterize the dynamic profile of nuclear and cytoplasmic transcripts during the progress of haemorrhage-induced neuroinflammation, we isolated nucleo-cytoplasmic RNA fractions of oxyhaemoglobin (oxy-Hb) treated microglia cells and sequenced both fractions. We discovered that cytoplasmic retained genes were the major forces to maintain the neuroinflammatory microenvironment with 10 hub genes and 40 conserved genes were identified. Moreover, antisense RNA Gm44096 and lincRNA Gm47270, which co-expressed with a crowd of inflammatory genes in the cytoplasm, were discovered as regulatory strategies for sustaining the neuroinflammatory microenvironment. Thus, our study provides a new perspective on understanding haemorrhage-induced neuroinflammation and also reveals a mechanism of lncRNA responsible for maintaining the neuroinflammatory microenvironment.</p>
Cell Biology
Genetics
Molecular Biology
Physiology
Immunology
Biological Sciences not elsewhere classified
Developmental Biology
Mental Health
Infectious Diseases
Plant Biology
Computational Biology
Taylor & Francis
2021
2021-11-29
2024-02-16
Dataset
50820398 Bytes
10.6084/m9.figshare.17092355
10.1080/15476286.2021.2004684
CC BY 4.0