10.6084/M9.FIGSHARE.16877124
Mingwang Long
Mingwang
Long
Yue Pan
Yue
Pan
Junying Chen
Junying
Chen
Fan Jia
Fan
Jia
Han Wang
Han
Wang
Daiying Li
Daiying
Li
Kai Feng
Kai
Feng
Lingmei Yan
Lingmei
Yan
Xiaodan Wang
Xiaodan
Wang
Xuelei Ning
Xuelei
Ning
Lijuan Qiu
Lijuan
Qiu
Juan Zhang
Juan
Zhang
Qiangming Sun
Qiangming
Sun
Sweeping analysis of transcript profile in dengue virus serotype 3 infection and antibody-dependent enhancement of infection
<p>Dengue virus infection mainly causes dengue hemorrhagic fever (DHF) and/or dengue shock syndrome (DSS). However, ADE (antibody-dependent enhancement) is one of the main pathogenic factors, and its pathogenic mechanism has not been fully elucidated. Recently, with the development of high-throughput sequencing, an increased number of RNAs have been confirmed to play a vital regulatory role in the process of virus infection. However, there is a lack of research on dengue virus infection and ADE. In this study, we used RNA-Seq to detect differentially expressed RNAs (DE RNAs) profiles in mock-infected, DENV-3-infected, and ADE-infected THP-1 cells. Firstly, we found 69 circRNAs, 259 miRNAs, and 18 mRNAs were differentially expressed in THP-1 vs DENV-3. In THP-1 vs ADE, 94 circRNAs, 263 miRNAs, and 111 mRNAs were differentially expressed. In DENV-3 vs ADE, 68 circRNAs, 105 miRNAs, and 94 mRNAs were differentially expressed. Functional enrichment analysis of these DE RNAs mainly focused on immune system, viral infectious diseases, cytokine-cytokine receptor interactions, and NOD/RIG-I-like receptor signaling pathways. In DENV-3 vs ADE, notably, the expression of HBB was up-regulated, which was a Fcγ Receptor-mediated phagocytosis protein. Additionally, we predicted the encoding ability of DE circRNAs, and it was found that a small peptide was encoded by novel_circ_001562 and that its amino acid sequence was consistent with that of DDX60L, which is a class of interferon-stimulated genes. Finally, we constructed the ceRNA regulatory network pathway. Therefore, our study provides a new strategy for further investigation on DENV-host interactions.</p>
Medicine
Microbiology
Molecular Biology
Immunology
Biological Sciences not elsewhere classified
Cancer
Mental Health
Infectious Diseases
Virology
Computational Biology
Taylor & Francis
2021
2021-11-08
2024-03-21
Dataset
9090461 Bytes
10.1080/21505594.2021.1996072
CC BY 4.0