10.6084/M9.FIGSHARE.16613797
Yi Luo
Yi
Luo
Xiu Qu
Xiu
Qu
Dan Kan
Dan
Kan
Binlin Cai
Binlin
Cai
The microRNA-451a/chromosome segregation 1-like axis suppresses cell proliferation, migration, and invasion and induces apoptosis in nasopharyngeal carcinoma
<p>MicroRNA-451a (miR-451a) has been implicated in the initiation and progression of multiple cancers. However, the regulatory mechanisms underlying its function in nasopharyngeal carcinoma (NPC) are poorly understood. Thus, we investigated in detail the role of the microRNA-451a/chromosome segregation 1-like (miR-45a/CSE1L) axis and its regulatory mechanism in NPC. We examined the levels of miR-451a and CSE1L in NPC, and assessed the effects of miR-451a and CSE1L on NPC by cell functional experiments. Furthermore, we elucidated the direct regulatory effect of miR-451a on <i>CSE1L</i> by the luciferase reporter assay, RNA pull-down assay, and RNA immunoprecipitation and validated our observations by calculating the Pearson’s correlation coefficient. We found that miR-451a was down-regulated in NPC cells, and its over-expression attenuated cell proliferation, migration, and invasion, and tumor growth in 5–8 F and SUNE-1 cells and promoted apoptosis. Moreover, <i>CSE1L</i> was the direct gene target of miR-451a, and its over-expression abrogated miR-451a-dependent inhibition of malignancy in 5–8 F and SUNE-1 cells. The Pearson’s correlation coefficient indicated a negative correlation between <i>CSE1L</i> and miR-451a. miR-451a serves as a tumor suppressor and targets <i>CSE1L</i>. miR-451a suppresses <i>CSE1L</i> expression, thereby reducing proliferation, invasion, and migration and increasing apoptosis of NPC cells.</p>
Biophysics
Biochemistry
Cell Biology
Genetics
Molecular Biology
Physiology
Environmental Sciences not elsewhere classified
Chemical Sciences not elsewhere classified
Biological Sciences not elsewhere classified
Cancer
Science Policy
Hematology
Taylor & Francis
2021
2021-12-31
2023-06-03
Dataset
803508 Bytes
10.1080/21655979.2021.1975018
CC BY 4.0