10.6084/M9.FIGSHARE.16613759
Yinyao Ma
Yinyao
Ma
Hua Wu
Hua
Wu
Xuxia Liang
Xuxia
Liang
Chun Zhang
Chun
Zhang
Yanhua Ma
Yanhua
Ma
Yanfen Wei
Yanfen
Wei
Jing Li
Jing
Li
Hui Chen
Hui
Chen
Identification of downstream targets and signaling pathways of long non-coding RNA NR_002794 in human trophoblast cells
<p>Preeclampsia (PE) is a huge threat to pregnant women. Our previous study demonstrated that long non-coding RNA (lncRNA) NR_002794 was highly expressed in placentas of PE patients and could regulate the phenotypes of trophoblast cells. However, the downstream regulatory mechanisms of NR_002794 remain unknown. In this text, some potential downstream targets or signaling pathways of NR_002794 were identified through RNA sequencing (RNA-seq) and bioinformatics analysis in SWAN71 trophoblast cells. Western blot assay demonstrated that NR_002794 inactivated protein kinase B (AKT) and extracellular signal-regulated kinase 1/2 (ERK1/2) pathways and activated cell apoptotic signaling in SWAN71 cells. Both RNA-seq and reverse transcription-quantitative PCR (RT-qPCR) outcomes showed that NR_002794 up-regulation could notably inhibit the expression of C-C motif chemokine ligand 4 like 2 (CCL4L2), interleukin 15 receptor subunit alpha (IL15RA), interleukin 32 (IL32), and tyrosine kinase with immunoglobulin-like and EGF-like domains 1 (TIE1), while NR_002794 knockdown induced these gene expressions in SWAN71 cells. CCK-8, BrdU, Transwell, wound healing, and flow cytometry analyses showed that NR_002794 inhibited cell proliferation and migration and induced cell apoptosis through down-regulating TIE1 in SWAN71 cells. In conclusion, lncRNA NR_002794 could exert its functions by regulating AKT and ERK1/2 pathways and TIE1 expression in human trophoblast cells.</p>
Biochemistry
Microbiology
Cell Biology
Genetics
Molecular Biology
Physiology
Pharmacology
Chemical Sciences not elsewhere classified
Immunology
Biological Sciences not elsewhere classified
Developmental Biology
Cancer
Hematology
Infectious Diseases
Virology
Taylor & Francis
2021
2021-12-31
2023-10-17
Dataset
285592 Bytes
10.1080/21655979.2021.1974808
CC BY 4.0