10.6084/M9.FIGSHARE.15141939
Zhongyan Zhao
Zhongyan
Zhao
Chanji Wu
Chanji
Wu
Xiangying He
Xiangying
He
Eryi Zhao
Eryi
Zhao
Shijun Hu
Shijun
Hu
Yeguang Han
Yeguang
Han
Ting Wang
Ting
Wang
Yanquan Chen
Yanquan
Chen
Tao Liu
Tao
Liu
Shixiong Huang
Shixiong
Huang
miR-152-3p aggravates vascular endothelial cell dysfunction by targeting DEAD-box helicase 6 (DDX6) under hypoxia
<p>Stroke is a main cause of disability and death worldwide, and ischemic stroke accounts for most stroke cases. Recently, microRNAs (miRNAs) have been verified to play critical roles in the development of stroke. Herein, we explored effects of miR-152-3p on vascular endothelial cell functions under hypoxia. Human umbilical vein endothelial cells (HUVECs) were treated with hypoxia to mimic cell injury <i>in vitro</i>. Reverse transcription quantitative polymerase chain reaction revealed that miR-152-3p exhibited high expression in HUVECs treated with hypoxia. The inhibition of miR-152-3p reversed hypoxia-induced decrease in cell viability and the increase in angiogenesis, according to the results of cell counting kit-8 assays and tube formation assays. miR-152-3p inhibition reversed the increase in endothelial cell permeability mediated by hypoxia, as shown by endothelial cell permeability <i>in vitro</i> assays. In addition, the increase in protein levels of angiogenetic markers and the decrease in levels of tight junction proteins induced by hypoxia were reversed by miR-152-3p inhibition. Mechanistically, miR-152-3p directly targets 3สน-untranslated region of DEAD-box helicase 6 (DDX6), which was confirmed by luciferase reporter assays. DDX6 is lowly expressed in HUVECs under hypoxic condition, and mRNA expression and protein level of DDX6 were upregulated in HUVECs due to miR-152-3p inhibition. Rescue assays showed that DDX6 knockdown reversed effects of miR-152-3p on cell viability, angiogenesis and endothelial permeability. The results demonstrated that miR-152-3p aggravates vascular endothelial cell dysfunction by targeting DDX6 under hypoxia.</p>
Biochemistry
Medicine
Cell Biology
Genetics
Molecular Biology
Physiology
Pharmacology
Chemical Sciences not elsewhere classified
Immunology
Marine Biology
Cancer
Hematology
Taylor & Francis
2021
2021-08-10
2023-06-02
Dataset
9541 Bytes
10.1080/21655979.2021.1959864
CC BY 4.0