10.6084/M9.FIGSHARE.15105232.V1
Yuan Tan
Yuan
Tan
Zhenglan Huang
Zhenglan
Huang
Xin Wang
Xin
Wang
Hongdan Dai
Hongdan
Dai
Guoyun Jiang
Guoyun
Jiang
Wenli Feng
Wenli
Feng
A novel fusion circular RNA F-circBA1 derived from the <i>BCR-ABL</i> fusion gene displayed an oncogenic role in chronic myeloid leukemia cells
<p>The <i>BCR-ABL</i> fusion gene plays a crucial role in the leukemogenesis of chronic myeloid leukemia (CML). The BCR-ABL oncoprotein encoded by this fusion gene has been extensively studied. However, research on whether <i>BCR-ABL</i> also affects circular RNAs (circRNAs) is limited. This study aimed to explore the new fusion circRNAs produced by <i>BCR-ABL</i> and their role in CML cells. In this study, we identified a novel fusion circRNA, named F-circBA1, originating from <i>BCR-ABL</i> in K562 and K562/G01 cells using quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) and Sanger sequencing. qRT-PCR of the nuclear RNA and cytoplasmic RNA were separated, indicating that F-circBA1 was mainly localized in the cytoplasm. Cell counting kit-8 assay and flow cytometry showed that F-circBA1 knockdown by shRNA prevented the proliferation of K562 and K562/G01 cells, and the cell cycle was arrested at G2/M. Mechanically, dual-luciferase reporter assay and western blotting assay showed that F-circBA1 sponged miR-148-3p and F-circBA1 silencing decreased CDC25B expression in vitro. Furthermore, the results of the murine leukemogenesis model showed that F-circBA1 knockdown suppressed leukemogenesis in vivo. Besides, we found the existence of F-circBA1 in some patients with <i>BCR-ABL</i>-positive CML. In conclusion, these results demonstrate the presence of F-circBA1 and its oncogenic role in CML cells.</p>
Biophysics
Biochemistry
Cell Biology
Molecular Biology
Cancer
Hematology
Taylor & Francis
2021
2021-08-04
2024-02-23
Dataset
29386970 Bytes
10.6084/m9.figshare.15105232
10.1080/21655979.2021.1957749
CC BY 4.0