10.6084/M9.FIGSHARE.14938688.V1
Yang Wang
Yang
Wang
Nan Lou
Nan
Lou
Min Zuo
Min
Zuo
Fuqiang Zhu
Fuqiang
Zhu
Yan He
Yan
He
Zhiqiang Cheng
Zhiqiang
Cheng
Xiaomei Wang
Xiaomei
Wang
STAT3-induced ZBED3-AS1 promotes the malignant phenotypes of melanoma cells by activating PI3K/AKT signaling pathway
<p>Melanoma is considered as the most frequent primary malignancy occurring in skin. Accumulating studies have suggested that long non-coding RNAs (lncRNAs) play critical parts in multiple cancers. In this study, we explored the molecular mechanism of ZBED3 antisense RNA 1 (ZBED3-AS1) in melanoma. We observed that ZBED3-AS1 expression was remarkably up-regulated in melanoma tissues, and high ZBED3-AS1 level was linked to unsatisfactory survival of melanoma patients. Then, we discovered that ZBED3-AS1 was overexpressed in melanoma cells compared with human epidermal melanocytes. In addition, loss-of-function assays verified that ZBED3-AS1 knockdown restrained cell proliferation, migration, epithelial–mesenchymal transition (EMT), and stemness in melanoma. In addition, signal transducer and activator of transcription 3 (STAT3), which also showed tumour-facilitating functions in melanoma, was confirmed as a transcriptional activator of ZBED3-AS1. Moreover, ZBED3-AS1 enhanced the expression of AT-rich interaction domain 4B (ARID4B) through sequestering miR-381-3p. Importantly, we further confirmed that ZBED3-AS1 promoted the malignant progression of melanoma by regulating miR-381-3p/ARID4B axis to activate the phosphatidylinositol 3-kinase/AKT serine/threonine kinase (PI3K/AKT) signalling pathway. In a word, our research might provide a novel therapeutic target for melanoma.</p>
Cell Biology
Genetics
Molecular Biology
Immunology
Biological Sciences not elsewhere classified
Developmental Biology
Cancer
Taylor & Francis
2021
2021-07-09
2024-03-21
Dataset
62809120 Bytes
10.6084/m9.figshare.14938688
10.1080/15476286.2021.1950463
CC BY 4.0