10.6084/M9.FIGSHARE.14480879.V1
Junping Guo
Junping
Guo
Wei Chen
Wei
Chen
Beibei Bao
Beibei
Bao
Dayong Zhang
Dayong
Zhang
Jianping Pan
Jianping
Pan
Mao Zhang
Mao
Zhang
Protective effect of berberine against LPS-induced endothelial cell injury via the JNK signaling pathway and autophagic mechanisms
<p>The role of autophagic mechanisms in the protective effect of berberine (BBR) on lipopolysaccharide (LPS)-induced injury in the endothelial cells human umbilical vein endothelial cells (HUVECs) and human pulmonary microvascular endothelial cells (HPMECs) was investigated. Cell viability, proliferation, and apoptosis were detected by the CCK-8 assay, the EdU kit, and flow cytometry, respectively, and autophagy-related protein expression, the number of autophagic vacuoles, and LC3 double-fluorescence were examined using western blot analysis, transmission electron microscopy, and confocal microscopy, respectively. LPS resulted in a decrease in the cell viability and proliferation of HUVECs and HPMECs and an increase in the number of apoptotic cells, while BBR treatment resulted in an increase in cell viability and proliferation, as well as a decrease in cell apoptosis. Furthermore, BBR could inhibit LPS-induced autophagy, as demonstrated by its inhibitory effects on the LC3-II/LC3-I ratio and Beclin-1 levels and its promotive effect on p62 expression. Addition of the autophagy inducer rapamycin (RAPA) aggravated LPS-induced injury, while treatment with the autophagy blocker 3-methyladenine (3-MA) attenuated the injury. Further, the protective effect of BBR was inhibited by rapamycin. JNK inhibition by SP600125 inhibited LPS-induced autophagy, and BBR could not alter the LPS-induced autophagy in HUVECs and HPMECs that were pretreated with SP600125. The present data indicate that BBR attenuated LPS-induced cell apoptosis by blocking JNK-mediated autophagy in HUVECs and HPMECs. Therefore, the JNK-mediated autophagy pathway could be a potential target for the prevention and treatment of cardiovascular disease.</p>
Biochemistry
Microbiology
Cell Biology
Genetics
Molecular Biology
Physiology
Pharmacology
Chemical Sciences not elsewhere classified
Immunology
Biological Sciences not elsewhere classified
Cancer
Hematology
Taylor & Francis
2021
2021-04-25
2024-02-19
Dataset
810112 Bytes
10.6084/m9.figshare.14480879
10.1080/21655979.2021.1915671
CC BY 4.0