10.6084/M9.FIGSHARE.14343371.V1
Yutao Wang
Yutao
Wang
Huixiang Zhang
Huixiang
Zhang
Jing Wang
Jing
Wang
Ming Yu
Ming
Yu
Qianqian Zhang
Qianqian
Zhang
Shan Yan
Shan
Yan
Dingyun You
Dingyun
You
Lanlan Shi
Lanlan
Shi
Lihuan Zhang
Lihuan
Zhang
Limei Wang
Limei
Wang
Hongxiang Wu
Hongxiang
Wu
Xue Cao
Xue
Cao
<i>Aconiti lateralis Radix Praeparata</i> inhibits Alzheimer’s disease by regulating the complex regulation network with the core of <i>GRIN1</i> and <i>MAPK1</i>
<p>Current medicine for Alzheimer’s disease (AD) cannot effectively reverse or block nerve injury. Traditional Chinese Medicine practice and research imply <i>Aconiti lateralis Radix Praeparata</i> (Fuzi) may meet this goal.</p> <p>Analysing the anti-AD effect of Fuzi and its potential molecular mechanism.</p> <p>AD model cells were treated with Fuzi in 0-300 mg/mL for 24 h in 37 °C. The cell viability (CV) and length of cell projections (LCP) for each group were observed, analysed, and standardised using control as a baseline (CV<sub>s</sub> and LCP<sub>s</sub>). The Fuzi and AD relevant genes were identified basing on databases, and the molecular mechanism of Fuzi anti-AD was predicted by network analysis.</p> <p>Experiment results showed that Fuzi in 0.4 mg/mL boosted LCP (LCP<sub>s</sub> = 1.2533, <i>p</i> ≤ 0.05), and in 1.6–100 mg/mL increased CV (CV<sub>s</sub> from 1.1673 to 1.3321, <i>p</i> ≤ 0.05). Bioinformatics analysis found 17 Fuzi target genes (relevant scores ≥ 20), showing strong AD relevant signals (RMS_<i>p</i> ≤ 0.05, related scores ≥ 5), enriched in the pathways regulating axon growth, synaptic plasticity, cell survival, proliferation, apoptosis, and death (<i>p</i> ≤ 0.05). Especially, <i>GRIN1</i> and <i>MAPK1</i> interacted with <i>APP</i> protein and located in the key point of the “Alzheimer’s disease” pathway.</p> <p>These results suggest that Fuzi may have therapeutic and prevention potential in AD, and <i>GRIN1</i> and <i>MAPK1</i> may be the core of the pathways of the Fuzi anti-AD process. Fuzi should be studied more extensively, especially for the prevention of AD.</p>
Biochemistry
Microbiology
Cell Biology
Molecular Biology
Neuroscience
Biotechnology
Chemical Sciences not elsewhere classified
Immunology
Biological Sciences not elsewhere classified
Mental Health
Taylor & Francis
2021
2021-03-30
2024-03-21
Online resource
0 Bytes
10.6084/m9.figshare.14343371
10.1080/13880209.2021.1900879
CC BY 4.0