10.6084/M9.FIGSHARE.13624046.V2
Patrick Ostheim
Patrick
Ostheim
University of Ulm
Alan Don Mallawaratchy
Alan Don
Mallawaratchy
Thomas Müller
Thomas
Müller
University Hospital Regensburg
Friedrich-Loeffler-Institut
University of Bern
Simone Schüle
Simone
Schüle
University of Ulm
Cornelius Hermann
Cornelius
Hermann
University of Ulm
Tanja Popp
Tanja
Popp
Stefan Eder
Stefan
Eder
University of Ulm
Stephanie E. Combs
Stephanie E.
Combs
Helmholtz Zentrum München
Technical University of Munich
German Cancer Research Center
Matthias Port
Matthias
Port
University of Ulm
Michael Abend
Michael
Abend
University of Ulm
Acute radiation syndrome-related gene expression in irradiated peripheral blood cell populations
<p>In a nuclear or radiological event, an early diagnostic tool is needed to distinguish the worried well from those individuals who may later develop life-threatenFing hematologic acute radiation syndrome. We examined the contribution of the peripheral blood's cell populations on radiation-induced gene expression (GE) changes.</p> <p>EDTA-whole-blood from six healthy donors was X-irradiated with 0 and 4Gy and T-lymphocytes, B-lymphocytes, NK-cells and granulocytes were separated using immunomagnetic methods. GE were examined in cell populations and whole blood.</p> <p>The cell populations contributed to the total RNA amount with a ratio of 11.6 for T-lymphocytes, 1.2 for B-cells, 1.2 for NK-cells, 1.0 for granulocytes. To estimate the contribution of GE per cell population, the baseline (0Gy) and the radiation-induced fold-change in GE relative to unexposed was considered for each gene. The T-lymphocytes (74.8%/80.5%) contributed predominantly to the radiation-induced up-regulation observed for <i>FDXR/DDB2</i> and the B-lymphocytes (97.1%/83.8%) for down-regulated <i>POU2AF1/WNT3</i> with a similar effect on whole blood gene expression measurements reflecting a corresponding order of magnitude.</p> <p>T- and B-lymphocytes contributed predominantly to the radiation-induced up-regulation of <i>FDXR/DDB2</i> and down-regulation of <i>POU2AF1/WNT3</i>. This study underlines the use of <i>FDXR/DDB2</i> for biodosimetry purposes and <i>POU2AF1/WNT3</i> for effect prediction of acute health effects.</p>
Biophysics
Medicine
Cell Biology
Genetics
Molecular Biology
59999 Environmental Sciences not elsewhere classified
Immunology
69999 Biological Sciences not elsewhere classified
Cancer
Hematology
Taylor & Francis
2021
2021-03-03
2023-01-06
Dataset
4454987 Bytes
10.1080/09553002.2021.1876953
10.6084/m9.figshare.13624046
CC BY 4.0