10.6084/M9.FIGSHARE.13385124.V1
Zhu Zeng
Zhu
Zeng
Haixin Ma
Haixin
Ma
Jing Chen
Jing
Chen
Nina Huang
Nina
Huang
Yudan Zhang
Yudan
Zhang
Yufei Su
Yufei
Su
Huifang Zhang
Huifang
Zhang
Knockdown of miR-1275 protects against cardiomyocytes injury through promoting neuromedin U type 1 receptor
<p>The present study aimed to assess the role of miR-1275 in cardiac ischemia reperfusion injury. H9 human embryonic stem cell (hESC)-derived cardiomyocytes stimulated by oxygen-glucose deprivation/reoxygenation (OGD/R) were used to simulate myocardial injury <i>in vitro</i>. miR-1275 expression levels in cells were measured by RT-qPCR. The release of lactate dehydrogenase (LDH) and creatine kinase (CK) was examined through LDH and CK ELISA kits. Cell apoptosis was detected through flow cytometry. A Fura-2 Calcium Flux Assay Kit and a Fluo-4 assay kit were used to determine the Ca<sup>2+</sup> concentration. Expression levels of proteins were tested by Western blotting. The binding effect of miR-1275 and neuromedin U type 1 receptor (NMUR1) was detected by dual-luciferase activity assay. The results showed that miR-1275 was upregulated in OGD/R-stimulated cardiomyocytes. Inhibition of miR-1275 suppressed the increased activity of LDH and CK, cell apoptosis, reactive oxygen species (ROS) production, intracellular Ca<sup>2+</sup> concentration and sarcoplasmic reticulum (SR) Ca<sup>2+</sup> leak induced by OGD/R treatment in cardiomyocytes. miR-1275 directly targets 3ʹUTR of NMUR1 and negatively regulates NMUR1 expression. Silence of NMUR1 abolished the protecting effect of the miR-1275 antagomir on myocardial OGD/R injury. Our study indicated that the miR-1275 antagomir protects cardiomyocytes from OGD/R injury through the promotion of NMUR1.</p>
Biochemistry
Cell Biology
Genetics
Molecular Biology
Physiology
Pharmacology
Chemical Sciences not elsewhere classified
Immunology
Cancer
Taylor & Francis
2020
2020-12-16
2024-02-15
Journal contribution
549429 Bytes
10.6084/m9.figshare.13385124
10.1080/15384101.2020.1860310
CC BY 4.0