10.6084/M9.FIGSHARE.13359562
Ying Hua
Ying
Hua
Xiangning Bai
Xiangning
Bai
Ji Zhang
Ji
Zhang
Cecilia Jernberg
Cecilia
Jernberg
Milan Chromek
Milan
Chromek
Sverker Hansson
Sverker
Hansson
Anne Frykman
Anne
Frykman
Xi Yang
Xi
Yang
Yanwen Xiong
Yanwen
Xiong
Chengsong Wan
Chengsong
Wan
Andreas Matussek
Andreas
Matussek
Molecular characteristics of <i>eae</i>-positive clinical Shiga toxin-producing <i>Escherichia coli</i> in Sweden
<p>Shiga toxin (Stx)-producing <i>Escherichia coli</i> (STEC) can cause a wide range of symptoms from asymptomatic carriage, mild diarrhea to bloody diarrhea (BD) and hemolytic uremic syndrome (HUS). Intimin, encoded by the <i>eae</i> gene, also plays a critical role in STEC pathogenesis. Herein, we investigated the prevalence and genetic diversity of <i>eae</i> among clinical STEC isolates from patients with diarrhea, BD, HUS as well as from asymptomatic STEC-positive individuals in Sweden with whole-genome sequencing. We found that 173 out of 239 (72.4%) of clinical STEC strains were <i>eae</i> positive. Six <i>eae</i> subtypes (<i>ϵ</i>1, <i>γ</i>1, <i>β</i>3, <i>θ</i>, <i>ζ</i> and <i>ρ</i>) were identified <i>eae</i> and its subtype <i>γ</i>1 were significantly overrepresented in O157:H7 strains isolated from BD and HUS patients. <i>ϵ</i>1 was associated with O121:H19 and O103:H2 strains, and <i>β</i>3 to O26:H11 strains. The combination of <i>eae</i> subtype <i>γ</i>1 and <i>stx</i> subtype (<i>stx</i><sub>2</sub> or <i>stx</i><sub>1</sub>+<i>stx</i><sub>2</sub>) is more likely to cause severe disease, suggesting the possibility of using <i>eae</i> genotypes in risk assessment of STEC infection. In summary, this study demonstrated a high prevalence of <i>eae</i> in clinical STEC strains and considerable genetic diversity of <i>eae</i> in STEC strains in Sweden from 1994 through 2018, and revealed association between <i>eae</i> subtypes and disease severity.</p>
Space Science
Medicine
Microbiology
Genetics
Molecular Biology
Neuroscience
Environmental Sciences not elsewhere classified
Biological Sciences not elsewhere classified
Cancer
Infectious Diseases
Virology
Taylor & Francis
2020
2020-12-10
2023-06-01
Dataset
62570 Bytes
10.1080/22221751.2020.1850182
CC BY 4.0