10.6084/M9.FIGSHARE.13029036.V1
Ting Yang
Ting
Yang
Tianhong Xie
Tianhong
Xie
Hua Li
Hua
Li
Xia Song
Xia
Song
Lei Yue
Lei
Yue
Xi Wang
Xi
Wang
Dong Shen
Dong
Shen
Kaili Ma
Kaili
Ma
Qinfang Jiang
Qinfang
Jiang
Runxiang Long
Runxiang
Long
Rong Yang
Rong
Yang
Xin He
Xin
He
Ye Zhang
Ye
Zhang
Zhongping Xie
Zhongping
Xie
Qihan Li
Qihan
Li
Immune responses of a CV-A16 live attenuated candidate strain and its protective effects in rhesus monkeys
<p>Coxsackievirus A16 (CV-A16) is a major causative pathogen of hand, foot, and mouth diseases (HFMDs). The licensed HFMD vaccine targets EV-A71 without cross-protection against CV-A16. Thus, a CV-A16 vaccine is needed. In this study, the immunogenicity and protective efficacy of a live attenuated CV-A16 candidate, K168-8Ac, were evaluated in a rhesus monkey model. Four passages of this strain (P35, P50, P60, and P70) were administered to monkeys, and its protective effect was identified. The immunized monkeys were clinically asymptomatic, except for slight fever. Weak viraemia was observed, and two doses of vaccination were found to significantly reduce virus shedding. High levels of antibody responses were observed (1:1024–1:2048), along with a significant increase in plasma IL-8. The I.M. group showed a much stronger humoural immunity. Pathological damage was detected mainly in lung tissues, although thalamus, spinal cord, lymph nodes, and livers were involved. After the viral challenge, it was found that two doses of vaccine reduced virus shedding, and the degree of lung damage and the number of organs involved decreased as the passage number increased. Overall, a robust immune response and partial protection against CV-A16, triggered by the K168-8Ac strain, were demonstrated. This study provides valuable data for CV-A16 vaccine development.</p>
Medicine
Physiology
Immunology
Biological Sciences not elsewhere classified
Infectious Diseases
Virology
Taylor & Francis
2020
2020-09-30
2024-02-28
Journal contribution
34816 Bytes
10.6084/m9.figshare.13029036
10.1080/22221751.2020.1823889
CC BY 4.0