10.6084/M9.FIGSHARE.11339450
De-Li ZhuGe
De-Li
ZhuGe
Li-Fen Wang
Li-Fen
Wang
Rui Chen
Rui
Chen
Xin-Ze Li
Xin-Ze
Li
Zhi-Wei Huang
Zhi-Wei
Huang
Qing Yao
Qing
Yao
Bin Chen
Bin
Chen
Ying-Zheng Zhao
Ying-Zheng
Zhao
He-Lin Xu
He-Lin
Xu
Jian-Dong Yuan
Jian-Dong
Yuan
Cross-linked nanoparticles of silk fibroin with proanthocyanidins as a promising vehicle of indocyanine green for photo-thermal therapy of glioma
<p>Instability of silk fibroin nanoparticles (SFNPs) in physiologic condition hinders its application as drug delivery vehicle. Herein, indocyanine green (ICG) loaded silk fibroin nanoparticles (ICG-SFNPs) was firstly prepared and then crosslinked by proanthocyanidins to obtain the stable ICG-CSFNPs for killing the residual tumour niche under near infra-red irradiation (NIR) after surgery. The particle size and zeta potentials of ICG-CSFNPs was 120.1 nm and -40.4 mV, respectively. Moreover, ICG-CSFNPs exhibited good stability of particle size in the physiological medium. Meanwhile, the stable photothermal properties of ICG-CSFNPs were not compromised even after several cycles of NIR. Few of the ICG-CSFNPs were phagocytized by RAW264.7 macrophage <i>in vitro</i>, while they were easily internalized by C6 glioma cells, resulting in their significant toxicity on tumour cells after NIR. The pharmacokinetic study showed that ICG-CSFNPs had a longer blood circulation time than ICG-SFNPs, making them more distribution in glioma after intravenous administration <i>in vivo</i>. Meanwhile, the pharmacological study showed the more effective inhibition of tumour growth was exhibited by ICG-CSFNPs in C6 glioma-bearing mice after NIR. Overall, the cross-linked nanoparticles of silk fibroin may be a promising vehicle of ICG for photothermal therapy of glioma after surgical resection.</p>
Biochemistry
Medicine
Cell Biology
Pharmacology
Biotechnology
Chemical Sciences not elsewhere classified
Mathematical Sciences not elsewhere classified
Cancer
Hematology
Taylor & Francis
2019
2019-12-07
2024-02-16
Journal contribution
324096 Bytes
10.1080/21691401.2019.1699819
CC BY 4.0