10.5518/1222
Hancock, Ashley
Ashley
Hancock
https://orcid.org/0000-0003-2069-5105
University of Leeds
Swainsbury, David
David
Swainsbury
https://orcid.org/0000-0002-0754-0363
University of Sheffield
Meredith, Sophie
Sophie
Meredith
https://orcid.org/0000-0003-2550-9092
University of Leeds
Morigaki, Kenichi
Kenichi
Morigaki
https://orcid.org/0000-0002-2454-6513
Kobe University
Hunter, Christopher
Christopher
Hunter
https://orcid.org/0000-0003-2533-9783
University of Sheffield
Adams, Peter
Peter
Adams
https://orcid.org/0000-0002-3940-8770
University of Leeds
Dataset for the study of Enhancing the spectral range of plant and bacterial Light-Harvesting pigment-protein complexes with various synthetic chromophores incorporated into lipid vesicles
University of Leeds
2022
dataset
10.1101/2022.08.02.502557
10.1016/j.jphotobiol.2022.112585
Creative Commons Attribution 4.0 International
The Light-Harvesting (LH) pigment-protein complexes found in photosynthetic organisms have the role of absorbing solar energy with high efficiency and transferring it to reaction centre complexes. LH complexes contain a suite of pigments that each absorb light at specific wavelengths, however, the natural combinations of pigments within any one protein complex do not cover the full range of solar radiation. Here, we provide an in-depth comparison of the relative effectiveness of five different organic “dye” molecules (Texas Red, ATTO, Cy7, Dil, DiR) for enhancing the absorption range of two different LH membrane protein complexes (the major LHCII from plants and LH2 from purple phototrophic bacteria). Proteoliposomes were self-assembled from defined mixtures of lipids, proteins and dye molecules and their optical properties were quantified by absorption and fluorescence spectroscopy. Both lipid-linked dyes and alternative lipophilic dyes were found to be effective excitation energy donors to LH protein complexes, without the need for direct chemical or generic modification of the proteins. The Förster theory parameters (e.g., spectral overlap) were compared between each donor-acceptor combination and found to be good predictors of an effective dye-protein combination. At the highest dye-to-protein ratios tested (over 20:1), the effective absorption strength integrated over the full spectral range was increased to ~180% of its natural level for both LH complexes. Lipophilic dyes could be inserted into pre-formed membranes although their effectiveness was found to depend upon favourable physicochemical interactions. Finally, we demonstrated that these dyes can also be effective at increasing the spectral range of surface-supported models of photosynthetic membranes, using fluorescence microscopy. The results of this work provide insight into the utility of self-assembled lipid membranes and the great flexibility of LH complexes for interacting with different dyes.
Engineering and Physical Sciences Research Council
https://doi.org/10.13039/501100000266
1807029, EP/T013958/1, EP/J017566/1, EP/T013958/1
Biotechnology and Biological Sciences Research Council
https://doi.org/10.13039/501100000268
B/M000265/1, BB/R000174/1
Japan Society for the Promotion of Science
https://doi.org/10.13039/501100001691
JPJSBP120195707, 19H04725, 21KK0088
European Research Council
https://doi.org/10.13039/501100000781
854126