10.5452/MA-AYO3R
Rose, Timothy Michael; Horst, Jeremy
KSHV glycoprotein B
modelarchive.org
2016
3D macromolecular model
2016-03-23
en
The glycoprotein B (gB) of the Kaposi's sarcoma-associated herpesvirus (KSHV) shows strong sequence homology to the Epstein-Barr Virus (EBV) gB homolog, including the conservation of 10 cysteines forming 5 critical disulphide-linkages. The crystal structure of EBV gB ectodomain (3fvc) has been determined, and was found to have a similar architecture to the gB of herpes simplex virus (Heldwein et al., 2006) and the G protein of vesicular stomatitus virus (Roche et al., 2006), which have been classified as class III viral fusion proteins. We generated top-scoring homology model structures of KSHV gB from the crystal structure of EBV gB (Backovic et al., 2009) using MODELLER, RAMP and ROSETTA programs. This model was estimated to be of high quality <4.1 ± 0.9 Angstroms all atom RMSD. The EBV and HSV1 gB homologs form trimer structures with three subunits wrapping around each other through multiple contact surfaces (Backovic et al., 2009; Heldwein et al., 2006). Model structures of the KSHV gB ectodomain monomer and trimer were determined.