10.5443/705
Suzanne Simard
Julie R. Deslippe
E.G. Farrell
Gaius Shaver
Martin Hartmann
William Mohn
Automated Ribosomal Inergenic Spacer Analysis (ARISA) of fungal Internal Transcribed Spacers region of 18S rRNA genes. Soil samples taken from Long-term Ecological Research (LTER) greenhouse treated and control plots at Toolik Lake, AK; 5 sampling da
Canadian Cryospheric Information Network
2016
DNA
Fungi
Genetic analysis
Soil microbiology
Soil temperature
Temperature
Tundra
International Polar Year-Carbon-flux and plant-microbial community dynamics in Low-Arctic tundra
Canadian Polar Data Network (CPDN)
IPY
1916-07-15/2007-08-23
en
705
Limited
In the summer of 2006 and 2007, we collected soils treated with warming by greenhouses since 1989, which are maintained as part of the Arctic Long-term Ecological Research (LTER) site at Toolik Lake, AK (68o38'N, 149o34'W, elevation 760m). Total nucleic acids were extracted from soil samples allowing for the simultaneous assessment of the total and actively growing components of the soil microbial community. We used Automated Ribosomal Inergenic Spacer Analysis (ARISA) of the Internal Transcribed Spacer (ITS) region of 18S rRNA genes to profile the fungal community.
Purpose: Assessment of long-term warming on structure and function of soil microbial communities.
Summary: Not Applicable
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Toolik Lake Alaska, USA (68o38'N, 149o34'W)