10.5285/A15C35D7-0247-4EA3-820B-F6F20CC5FFED
Glanville, H.C.
https://orcid.org/0000-0002-8192-6678
Bangor University
De Sosa, L.L.
Bangor University
Marshall, M.R.
https://orcid.org/0000-0003-2869-0616
Centre for Ecology & Hydrology
Cooper, D.M.
https://orcid.org/0000-0001-7578-7918
Centre for Ecology & Hydrology
Jones, D.L.
Bangor University
Time series of microbial carbon release from soil as carbon dioxide under different nitrogen and phosphorus treatments with a high glucose concentration added as a carbon source in the Conwy catchment, North Wales, UK (2016)
NERC Environmental Information Data Centre
2018
Turf2Surf (T2S)
Macronutrient Cycling Programme (MCP)
Environmental survey
liquid scintillation counter
Soil core
Conwy Catchment
North Wales
Dr. Helen Glanville
Bangor University
NERC EDS Environmental Information Data Centre
https://ror.org/04xw4m193
2018-03-19
2016-11-30
2018-08-01
en
https://catalogue.ceh.ac.uk/id/a15c35d7-0247-4ea3-820b-f6f20cc5ffed
https://data-package.ceh.ac.uk/sd/a15c35d7-0247-4ea3-820b-f6f20cc5ffed.zip
text/csv Comma-separated values (CSV)
This resource is made available under the terms of the Open Government Licence
Time series data of carbon release in disintegrations per minute are presented for different nitrogen and phosphorus treatments with a high glucose concentration substrate added as a carbon source to soil samples from six depths (0-15, 15-30, 50-100, 100-150, 150-200 and 250-300 centimetres). Soil cores were collected from a field experiment in the Conwy catchment in July 2016 and returned the laboratories of the School of Environment, Natural Resources and Geography, Bangor University. A high molecular weight substrate was added as a carbon source to the samples and the rate of 14C-substrate mineralization measured.
All the work was carried out by trained members of staff from Bangor University and the Centre for Ecology & Hydrology.
The measurements were taken to improve understanding of the relationship between microbial activity and soil properties and depth of sampling, under differing nutrient availability.
The data were collected for the NERC project 'The Multi-Scale Response of Water quality, Biodiversity and Carbon Sequestration to Coupled Macronutrient Cycling from Source to Sea' (NE/J011991/1). The project is also referred to as Turf2Surf.
Soil cores were collected from the field site and returned to laboratories of the School of Environment, Natural Resources and Geography, Bangor University.
In the laboratory soil cores were divided into depth intervals of 0-15, 15-30, 50-100, 100-150, 150-200 and 250-300 centimetres and passed through a 5 millimetre sieve in order to remove stones and any plant material and to ensure sample homogeneity.
To measure the rate of 14C-substrate mineralization, 5 grams of soil (dry weight equivalent to account for soil water content variability down the soil profile) was placed into sterile 50 ml polypropylene tubes. To determine the rate of 14CO2 evolution, 50 microlitres (µl) of 14C-glucose labelled nutrient solution was added to the soil surface. Immediately after nutrient addition, a vial containing 1 millilitre (ml) Sodium Hydroxide (NaOH) trap (1 Molar (M)) was added into the polypropylene tubes to capture 14CO2 evolved. The tubes were hermetically sealed and incubated at 10 degrees Celsius to represent the mean annual temperature of the catchment. The NaOH traps were changed after 0.5, 1, 2, 4, 6, 24, 48, 72, 96, 120, 144, 168, 192, 336, 504, 672, 840 and 1008 hours and then weekly up to six weeks after initial 14C-labelling for the glucose-C additions. On removal, the NaOH traps were mixed with Optiphase HiSafe 3® liquid scintillation fluid (PerkinElmer Inc.) and the amount of 14CO2 captured was determined using a Wallac 1404 liquid scintillation counter (Wallac EG & G).
Data were entered into an Excel spreadsheet and exported as a .csv file for ingestion into the EIDC
-3.784
-3.781
53.201
53.204
Natural Environment Research Council
https://ror.org/02b5d8509
NE/J011991/1
The Multi-Scale Response of Water quality, Biodiversity and C Sequestration to Coupled Macronutrient Cycling from Source to Sea