10.5285/27DA4400-9835-4241-9DF9-A3B598F8D274
MacDonald, H.C.
Cardiff University
Bruford, M.W.
Cardiff University
Ormerod, S.
https://orcid.org/0000-0002-8174-302X
Cardiff University
Jones, T.H.
Cardiff University
Genotype data for three freshwater macroinvertebrate species in upland Wales, UK (2012-2013)
NERC Environmental Information Data Centre
2017
Diversity of Upland Rivers for Ecosystem Service Sustainability (DURESS)
Amphinemura sulcicollis
Isoperla grammatica
Baetis rhodani
Hannah MacDonald
Cardiff University
Cardiff University
https://ror.org/03kk7td41
NERC EDS Environmental Information Data Centre
https://ror.org/04xw4m193
2017-09-20
2013-06-15
en
https://catalogue.ceh.ac.uk/id/27da4400-9835-4241-9df9-a3b598f8d274
https://data-package.ceh.ac.uk/sd/27da4400-9835-4241-9df9-a3b598f8d274.zip
text/csv Comma-separated values (CSV)
This resource is made available under the terms of the Open Government Licence
The resource consists of genotype data (genetic constitution of an individual organism) for three freshwater macroinvertebrate species, Amphinemura sulcicollis, Isoperla grammatica and Baetis rhodani collected at sites in upland Wales during May 2012 and May 2013. New nucleotide tandem repeats in DNA sequences i.e. Novel microsatellites were developed for this project so that genetic analysis could be carried out within the three species. Extra data on how microsatellite scores were grouped i.e. binned into unique alleles is provided, to aid replication. The data was generated for Hannah Macdonald's PhD thesis: to study genetic structure and diversity, demographic species history and resilience in freshwater ecosystems.
The work was carried out under Diversity in Upland Rivers for Ecosystem Service Sustainability (DURESS) project (Grant reference NERC NE/J014818/1). DURESS was funded by the Natural Environment Research Council (NERC) under the Biodiversity and Ecosystem Service Sustainability (BESS) programme.
Samples of each invertebrate were collected by kicksampling in May in 2012 and 2013, samples were sorted and identified to genus level on site and stored in absolute ethanol. In the laboratory each larva was identified to species level using Elliott et al. (1988) and Hynes (1977). DNA was then extracted from tissue samples (see supporting information for extraction methods). Baetis rhodani samples contained cryptic species which had to be removed before genotyping, no cryptic species were identified for Amphinemura sulcicollis or Isoperla grammatica. Samples were sent for fragment analysis (Dundee Biosciences, UK) using ROX500 as the internal size standard. Fragment analysis products were visualised and scored using Genemarker v 1.91 (Holland and Parson 2011). These results were downloaded from Genemarker and allele sizes were binned by hand using the 'Binning' information (provided in the metadata) in an Excel spreadsheet. The data were then complied into master Excel spreadsheets for each invertebrate. The data were then exported as comma separated value files for ingestion into the EIDC.
-4.073
-3.08
51.921
53.123
Natural Environment Research Council
https://ror.org/02b5d8509
NE/J014818/1
Diversity in Upland Rivers for Ecosystem Service Sustainability - DURESS