10.5285/18023DB5-25D8-44F7-B291-61869F937367
Griffiths, R.
Centre for Ecology & Hydrology
Edwards, F.
Centre for Ecology & Hydrology
Scarlett, P.
Centre for Ecology & Hydrology
Webb, G.
Centre for Ecology & Hydrology
Sarlej, R.
Centre for Ecology & Hydrology
Thomson, B.
Centre for Ecology & Hydrology
Operational taxonomic units for epilithon eukaryotes in the rivers Wolf and Tamar catchments
NERC Environmental Information Data Centre
2014
Biodiversity
Environmental monitoring facilities
Species distribution
microbiology
Tellus
South West project
microbiology
soil
genetics
hydrography
Edwards, F.
UK Centre for Ecology & Hydrology
UK Centre for Ecology & Hydrology
https://ror.org/00pggkr55
NERC EDS Environmental Information Data Centre
https://ror.org/04xw4m193
2014-05-06
2010-01-01
en
CEH:EIDC:#1395928342078
https://catalogue.ceh.ac.uk/id/18023db5-25d8-44f7-b291-61869f937367
https://data-package.ceh.ac.uk/sd/18023db5-25d8-44f7-b291-61869f937367.zip
text/csv Comma-separated values (CSV)
This resource is made available under the terms of the Open Government Licence
This datset contains operational taxonomic units for epilithon eukaryotes (water samples): Approximate location of sampling sites was determined from maps to provide good spatial coverage of the Wold River through to the Tamar River. Exact sites were determined in the field, considering accessibility and other logistics. The exact location of each sample site was determined using a Garmin GPS12. Three stones were taken from each of the 20 locations and epilithon removed from a defined area. Samples were kept in the cold and removed to the laboratory for analyses. DNA was extracted from all soil and epilithon samples using the MOBIO Powersoil 96 well DNA extraction kit. DNA was quality checked for purity and yield prior to submission for 454 pyrosequencing to assess both bacterial and eukaryotic biodiversity within each sample. Following bioinformatic sequence processing, sequencing were clustered into operational taxonomic units (OTU) and the data tables display the percentage of each OTU within each sample.
Epilithon (water samples): Approximate location of sampling sites was determined from maps to provide good spatial coverage of the Wold River through to the Tamar River. Exact sites were determined in the field, considering accessibility and other logistics. The exact location of each sample site was determined using a Garmin GPS12. Three stones were taken from each of the 20 locations and epilithon removed from a defined area. Samples were kept in the cold and removed to the laboratory for analyses. DNA was extracted from all soil and epilithon samples using the MOBIO Powersoil 96 well DNA extraction kit. DNA was quality checked for purity and yield prior to submission for 454 pyrosequencing to assess both bacterial and eukaryotic biodiversity within each sample. Following bioinformatic sequence processing, sequencing were clustered into operational taxonomic units (OTU) and the data tables display the percentage of each OTU within each sample.
-6.452
1.768
49.864
55.812