10.5281/ZENODO.6597895
K. P. BASU
J. N. KARKUN
SUCCINIC ACID DEHYDROGENASE FROM CUCUMBER SEEEDS
Zenodo
1943
enzyme
isolation
1943-12-31
en
https://zenodo.org/record/6597896
10.5281/zenodo.6597896
Creative Commons Attribution 4.0 International
Open Access
<p>The preparation and properties<span> </span>of succinic acid dehydrogenase from cucumber seeds are described. Methylene blue cannot serve as hydrogen acceptor, but the enzyme is a perfect dehydrogenase as it can use a dye of higher pH(2 :6-dichlorophenolindophenol blue) as hydrogen acceptor. Narcotics inhibit the dehydrogenating property of the enzyme whereas potassium cyanide inhibits the power of utilising oxygen as hydrogen acceptor. Sodium fluoried inhibits the dehydrogenating factor considerably and the oxidise factor feebly. Pyrophosphate is inhibi­tory to both. Incubation for a certain interval with copper sulphate at a concentration, which is usually harmless to the enzyme, greatly inhibits the enzymic activity showing that the enzyme contains a -SH group in the protein molecule.</p>